TY - JOUR
T1 - Heterologous expression and functional analysis of the gene cluster for the biosynthesis of and immunity to the lantibiotic, nukacin ISK-1
AU - Aso, Yuji
AU - Nagao, Jun Ichi
AU - Koga, Hanako
AU - Okuda, Ken Ichi
AU - Kanemasa, Youhei
AU - Sashihara, Toshihiro
AU - Nakayama, Jiro
AU - Sonomoto, Kenji
N1 - Funding Information:
We thank F. Götz (the University of Tübingen) for providing S. carnosus TM300 and pCU1. This work was partially supported by Grants-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (JSPS) and the Novartis Foundation (Japan) for the Promotion of Science, a Sasakawa Scientific Research Grant from the Japan Science Society, and JSPS research fellowships.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2004/12
Y1 - 2004/12
N2 - Nukacin ISK-1 is a lantibiotic produced by Staphylococcus warneri ISK-1. The gene cluster of nukacin ISK-1 consists of at least nukAMTFEG, ORF1 and ORF7. In this study, we demonstrated the heterologous production of nukacin ISK-1 in Lactococcus lactis by the artificial polycistronic expression of nukAMTFEG-ORF7 under the control of the nisin-controlled expression (NICE) system. Consequently, the recombinant L. lactis showed antimicrobial activity. Mass analysis clarified the presence of nukacin ISK-1 produced in the culture supernatant. These results suggested that the recombinant L. l.actis produced nukacin ISK-1 heterologously. Inactivation of nukA, -M or -T resulted in the complete loss of the nukacin ISK-1 production phenotype. This finding suggested that nukAMT are indispensably associated with the biosynthesis of nukacin ISK-1. To our knowledge, this is the first report of the heterologous production of lantibiotic using the NICE system.
AB - Nukacin ISK-1 is a lantibiotic produced by Staphylococcus warneri ISK-1. The gene cluster of nukacin ISK-1 consists of at least nukAMTFEG, ORF1 and ORF7. In this study, we demonstrated the heterologous production of nukacin ISK-1 in Lactococcus lactis by the artificial polycistronic expression of nukAMTFEG-ORF7 under the control of the nisin-controlled expression (NICE) system. Consequently, the recombinant L. lactis showed antimicrobial activity. Mass analysis clarified the presence of nukacin ISK-1 produced in the culture supernatant. These results suggested that the recombinant L. l.actis produced nukacin ISK-1 heterologously. Inactivation of nukA, -M or -T resulted in the complete loss of the nukacin ISK-1 production phenotype. This finding suggested that nukAMT are indispensably associated with the biosynthesis of nukacin ISK-1. To our knowledge, this is the first report of the heterologous production of lantibiotic using the NICE system.
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U2 - 10.1016/S1389-1723(05)00308-7
DO - 10.1016/S1389-1723(05)00308-7
M3 - Article
C2 - 16233732
AN - SCOPUS:12444307516
SN - 1389-1723
VL - 98
SP - 429
EP - 436
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
IS - 6
ER -