Heterooligomer of type 1 and type 2 inositol 1, 4, 5-trisphosphate receptor expressed in rat liver membrane fraction exists as tetrameric complex

Hitoshi Onoue, Hiroyuki Tanaka, Kiyoshi Tanaka, Naoko Doira, Yushi Ito

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Functional IP3-sensitive intracellular Ca2+ release channel is considered to be a tetramer of IP3R. Heterooligomeric complexes composed of distinct types of IP3R have been reported, however, crucial evidences for them being tetramer have not appeared. Here we report that the heterooligomer composed of IP3R1 and IP3R2 also exists as tetramer. Cross-linked heterooligomer was immunoprecipitated with IP3RI-specific antibody and detected by agarose-PAGE/Western blot analysis with IP3R2-specific antibody. Tetramer, trimer, dimer, and possibly monomer were detected. The trimer, dimer, and monomer were likely to be originated from the tetramer, since: (1) the immunoprecipitating antibody (IP3R1-specific) does not recognize IP3R2, therefore IP3R2 monomer itself could not have been immunoprecipitated; and (2) tetramer was the major native product of IP3R complex containing type 2 isoform in liver membrane fraction. Thus we conclude tetramer is the native form of heterooligomer composed of IP3R1 and IP3R2. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)928-933
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume267
Issue number3
DOIs
Publication statusPublished - Jan 27 2000

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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