HGF/SF is present in normal adult skeletal muscle and is capable of activating satellite cells

Ryuichi Tatsumi, Judy E. Anderson, Cedrine J. Nevoret, Orna Halevy, Ronald E. Allen

Research output: Contribution to journalArticle

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Abstract

We have shown that hepatocyte growth factor/scatter factor can stimulate activation and early division of adult satellite cells in culture, and that the action of hepatocyte growth factor/scatter factor is similar to the action of the unidentified satellite cell activator found in extracts of crushed muscle. We now provide new evidence that hepatocyte growth factor/scatter factor is present in uninjured adult rat skeletal muscle and that the activating factor in crushed muscle extract is hepatocyte growth factor/scatter factor. Immunoblots of crushed muscle extract demonstrate the presence of hepatocyte growth factor/scatter factor. Furthermore, crushed muscle extract stimulates the scattering of cultured MDCK cells. Immunolocalization studies with adult rat skeletal muscle show the presence of hepatocyte growth factor/scatter factor in the extracellular matrix surrounding muscle fibers; in addition, the receptor for hepatocyte growth factor/scatter factor, c-met, is localized to putative satellite cells. In muscle from mdx mice, hepatocyte growth factor/scatter factor and c-met are colocalized in activated satellite cells in regions of muscle repair. Moreover, the satellite cell-activating activity of crushed muscle extract is abolished by preincubation with anti-hepatocyte growth factor antibodies. Finally, direct injection of hepatocyte growth factor/scatter factor into uninjured tibialis anterior muscle of 12-month-old rats stimulated satellite cell activation. These experiments demonstrate that hepatocyte growth factor/scatter factor is present in muscle, can be released upon injury, and has the ability to activate quiescent satellite cells in vivo.

Original languageEnglish
Pages (from-to)114-128
Number of pages15
JournalDevelopmental Biology
Volume194
Issue number1
DOIs
Publication statusPublished - Feb 1 1998
Externally publishedYes

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Hepatocyte Growth Factor
Skeletal Muscle
Muscles
Proto-Oncogene Proteins c-met
Inbred mdx Mouse
Madin Darby Canine Kidney Cells

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Cite this

HGF/SF is present in normal adult skeletal muscle and is capable of activating satellite cells. / Tatsumi, Ryuichi; Anderson, Judy E.; Nevoret, Cedrine J.; Halevy, Orna; Allen, Ronald E.

In: Developmental Biology, Vol. 194, No. 1, 01.02.1998, p. 114-128.

Research output: Contribution to journalArticle

Tatsumi, Ryuichi ; Anderson, Judy E. ; Nevoret, Cedrine J. ; Halevy, Orna ; Allen, Ronald E. / HGF/SF is present in normal adult skeletal muscle and is capable of activating satellite cells. In: Developmental Biology. 1998 ; Vol. 194, No. 1. pp. 114-128.
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