High-accuracy analysis system for the redox status of coenzyme Q 10 by online supercritical fluid extraction-supercritical fluid chromatography/mass spectrometry

Atsuki Matsubara, Kazuo Harada, Kazumasa Hirata, Eiichiro Fukusaki, Takeshi Bamba

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)

Abstract

The accurate profiling of oxidative stress markers is often disturbed by oxidation occurred during sample preparation. In this study, an online supercritical fluid extraction-supercritical fluid chromatography/mass spectrometry system was constructed utilizing supercritical fluid as an efficient solvent for extraction. A higher ratio of the reduced form of coenzyme Q 10 (CoQ 10) was detected by online extraction, rather than by offline extraction using a conventional organic solvent. The results suggest that the profile of easily oxidized compounds can be acquired with high accuracy using this system because the oxidation of the target compounds during sample preparation can be avoided when using the online extraction.

Original languageEnglish
Pages (from-to)76-79
Number of pages4
JournalJournal of Chromatography A
Volume1250
DOIs
Publication statusPublished - Aug 10 2012
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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