High albumin production by multicellular spheroids of adult rat hepatocytes formed in the pores of polyurethane foam

Taku Matsushita; Hiroyuki Ijima; Norio Koide; Kazumori Funatsu

doi:10.1007/BF00208150

High albumin production by multicellular spheroids of adult rat hepatocytes formed in the pores of polyurethane foam

Taku Matsushita, Hiroyuki Ijima, Norio Koide, Kazumori Funatsu

Molecular and Biochemical Systems Engineering

Research output: Contribution to journal › Article › peer-review

101 Citations (Scopus)

Abstract

Adult rat hepatocytes formed spherical multicellular aggregates (spheroids) when they were cultured in the pores of polyurethane foam (PUF). The diameter of the spheroids was within the range 100-200 μm. These spheroids partly attached and immobilized in the PUF pores for at least 2 weeks. The albumin production rate by the spheroids increased up to 17.0 μg/10⁶ nuclei per day during the first 6 days and maintained at a high level for 2 weeks. In contrast, the albumin production rate by the monolayer markedly decreased after 3 days. The spheroid culture using PUF seems to be a convenient and simple method for maintaining some differentiated functions of hepatocytes and for making a bioreactor using the function of spheroids.

Original language	English
Pages (from-to)	324-326
Number of pages	3
Journal	Applied Microbiology and Biotechnology
Volume	36
Issue number	3
DOIs	https://doi.org/10.1007/BF00208150
Publication status	Published - Dec 1 1991

All Science Journal Classification (ASJC) codes

Biotechnology
Applied Microbiology and Biotechnology

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10.1007/BF00208150

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title = "High albumin production by multicellular spheroids of adult rat hepatocytes formed in the pores of polyurethane foam",

abstract = "Adult rat hepatocytes formed spherical multicellular aggregates (spheroids) when they were cultured in the pores of polyurethane foam (PUF). The diameter of the spheroids was within the range 100-200 μm. These spheroids partly attached and immobilized in the PUF pores for at least 2 weeks. The albumin production rate by the spheroids increased up to 17.0 μg/106 nuclei per day during the first 6 days and maintained at a high level for 2 weeks. In contrast, the albumin production rate by the monolayer markedly decreased after 3 days. The spheroid culture using PUF seems to be a convenient and simple method for maintaining some differentiated functions of hepatocytes and for making a bioreactor using the function of spheroids.",

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AU - Matsushita, Taku

AU - Ijima, Hiroyuki

AU - Koide, Norio

AU - Funatsu, Kazumori

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N2 - Adult rat hepatocytes formed spherical multicellular aggregates (spheroids) when they were cultured in the pores of polyurethane foam (PUF). The diameter of the spheroids was within the range 100-200 μm. These spheroids partly attached and immobilized in the PUF pores for at least 2 weeks. The albumin production rate by the spheroids increased up to 17.0 μg/106 nuclei per day during the first 6 days and maintained at a high level for 2 weeks. In contrast, the albumin production rate by the monolayer markedly decreased after 3 days. The spheroid culture using PUF seems to be a convenient and simple method for maintaining some differentiated functions of hepatocytes and for making a bioreactor using the function of spheroids.

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