TY - JOUR
T1 - High Axial and Lateral Resolutions on Self-Assembled Gold Nanoparticle Metasurfaces for Live-Cell Imaging
AU - Masuda, Shihomi
AU - Kuboki, Thasaneeya
AU - Kidoaki, Satoru
AU - Lee, Shi Ting
AU - Ryuzaki, Sou
AU - Okamoto, Koichi
AU - Arima, Yusuke
AU - Tamada, Kaoru
N1 - Funding Information:
The authors thanks Y. Aida and Nikon Solutions Co., Ltd. for technical assistance. This work was supported by JSPS KAKENHI Grant Number 19H05627 in Japan. This work was performed under the Cooperative Research Program of the “Network Joint Research Center for Materials and Devices”. The plasmid with the Venus gene for live-cell imaging was kindly provided by Professor T. Nagai at The Institute of Scientific and Industrial Research, Osaka University, Japan.
Publisher Copyright:
©
PY - 2020/11/25
Y1 - 2020/11/25
N2 - A plasmonic metasurface composed of homogeneously self-assembled gold nanoparticles can provide high-contrast fluorescence images confined to the nanointerface. In this study, we successfully demonstrated real-time, high-spatiotemporal-resolution imaging of adhered Venus-paxillin-3T3 live cells under a widefield microscope, where not only a high axial resolution but also a high lateral resolution down to the theoretical limit were confirmed through nascent cluster formation of paxillin. The improved lateral resolution on the sheet could be interpreted as the characteristic of localized surface plasmon resonance (LSPR)-mediated enhanced fluorescence and the metasurface acting as a nanothickness plane light emitter. We also found minimized photobleaching, owing to the increase in the emission efficiency via plasmon-exciton coupling. This simple nanomaterial-based technique will be a powerful tool to enhance interfacial signals and improve the quality of live-cell images, not only under widefield microscopes but also in combination with various super-resolution microscope systems in the future.
AB - A plasmonic metasurface composed of homogeneously self-assembled gold nanoparticles can provide high-contrast fluorescence images confined to the nanointerface. In this study, we successfully demonstrated real-time, high-spatiotemporal-resolution imaging of adhered Venus-paxillin-3T3 live cells under a widefield microscope, where not only a high axial resolution but also a high lateral resolution down to the theoretical limit were confirmed through nascent cluster formation of paxillin. The improved lateral resolution on the sheet could be interpreted as the characteristic of localized surface plasmon resonance (LSPR)-mediated enhanced fluorescence and the metasurface acting as a nanothickness plane light emitter. We also found minimized photobleaching, owing to the increase in the emission efficiency via plasmon-exciton coupling. This simple nanomaterial-based technique will be a powerful tool to enhance interfacial signals and improve the quality of live-cell images, not only under widefield microscopes but also in combination with various super-resolution microscope systems in the future.
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U2 - 10.1021/acsanm.0c02300
DO - 10.1021/acsanm.0c02300
M3 - Article
AN - SCOPUS:85096140787
SN - 2574-0970
VL - 3
SP - 11135
EP - 11142
JO - ACS Applied Nano Materials
JF - ACS Applied Nano Materials
IS - 11
ER -