High expression of the Cap43 gene in infiltrating macrophages of human renal cell carcinomas

Akihiro Nishie, Katsuaki Masuda, Michihiro Otsubo, Toshiro Migita, Masazumi Tsuyenosi, Kimitoshi Kohno, Taro Shuin, Seiji Naito, Mayumi Ono, Michihiko Kuwano

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

We used suppression subtractive hybridization to identify highly expressed genes in the cancerous region of human renal cell carcinoma (RCC) compared with noncancerous tissue. Nine genes were identified to show increased expression in the cancerous region compared with the noncancerous region. The nine genes included thymosin β4, secreted protein acidic and rich in cysteine (SPARC), Cap43, ceruloplasmin, serum amyloid A, osteopontin, heat shock protein 90 (HSP90), LOT1, and casein kinase I. Of these 9 genes, in situ hybridization with 10 clinical samples consistently showed a strong expression of Cap43 mRNA in infiltrating macrophages in RCCs, but not in cancer cells proliferating in an alveolar pattern. However, Cap43 mRNA was also apparently detected in epithelial cells of the renal proximal tubuli in noncancerous tissue. The higher expression of the Cap43 gene in the cancerous region of RCCs appears to depend on macrophage infiltration. Moreover, treatment with phorbol ester resulted in enhanced expression of the Cap43 gene in human monocytic cells in vitro. The expression of the Cap43 gene in infiltrating macrophages is discussed in association with the differentiated or activated status of monocyte/macrophage.

Original languageEnglish
Pages (from-to)2145-2151
Number of pages7
JournalClinical Cancer Research
Volume7
Issue number7
Publication statusPublished - Jan 1 2001

Fingerprint

Renal Cell Carcinoma
Macrophages
Gene Expression
Genes
Casein Kinase I
Thymosin
HSP90 Heat-Shock Proteins
Serum Amyloid A Protein
Messenger RNA
Osteopontin
Ceruloplasmin
Phorbol Esters
In Situ Hybridization
Cysteine
Monocytes
Epithelial Cells
Kidney
Neoplasms
Proteins

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Nishie, A., Masuda, K., Otsubo, M., Migita, T., Tsuyenosi, M., Kohno, K., ... Kuwano, M. (2001). High expression of the Cap43 gene in infiltrating macrophages of human renal cell carcinomas. Clinical Cancer Research, 7(7), 2145-2151.

High expression of the Cap43 gene in infiltrating macrophages of human renal cell carcinomas. / Nishie, Akihiro; Masuda, Katsuaki; Otsubo, Michihiro; Migita, Toshiro; Tsuyenosi, Masazumi; Kohno, Kimitoshi; Shuin, Taro; Naito, Seiji; Ono, Mayumi; Kuwano, Michihiko.

In: Clinical Cancer Research, Vol. 7, No. 7, 01.01.2001, p. 2145-2151.

Research output: Contribution to journalArticle

Nishie, A, Masuda, K, Otsubo, M, Migita, T, Tsuyenosi, M, Kohno, K, Shuin, T, Naito, S, Ono, M & Kuwano, M 2001, 'High expression of the Cap43 gene in infiltrating macrophages of human renal cell carcinomas', Clinical Cancer Research, vol. 7, no. 7, pp. 2145-2151.
Nishie A, Masuda K, Otsubo M, Migita T, Tsuyenosi M, Kohno K et al. High expression of the Cap43 gene in infiltrating macrophages of human renal cell carcinomas. Clinical Cancer Research. 2001 Jan 1;7(7):2145-2151.
Nishie, Akihiro ; Masuda, Katsuaki ; Otsubo, Michihiro ; Migita, Toshiro ; Tsuyenosi, Masazumi ; Kohno, Kimitoshi ; Shuin, Taro ; Naito, Seiji ; Ono, Mayumi ; Kuwano, Michihiko. / High expression of the Cap43 gene in infiltrating macrophages of human renal cell carcinomas. In: Clinical Cancer Research. 2001 ; Vol. 7, No. 7. pp. 2145-2151.
@article{ed7bcbc3def744938b575c5319e56896,
title = "High expression of the Cap43 gene in infiltrating macrophages of human renal cell carcinomas",
abstract = "We used suppression subtractive hybridization to identify highly expressed genes in the cancerous region of human renal cell carcinoma (RCC) compared with noncancerous tissue. Nine genes were identified to show increased expression in the cancerous region compared with the noncancerous region. The nine genes included thymosin β4, secreted protein acidic and rich in cysteine (SPARC), Cap43, ceruloplasmin, serum amyloid A, osteopontin, heat shock protein 90 (HSP90), LOT1, and casein kinase I. Of these 9 genes, in situ hybridization with 10 clinical samples consistently showed a strong expression of Cap43 mRNA in infiltrating macrophages in RCCs, but not in cancer cells proliferating in an alveolar pattern. However, Cap43 mRNA was also apparently detected in epithelial cells of the renal proximal tubuli in noncancerous tissue. The higher expression of the Cap43 gene in the cancerous region of RCCs appears to depend on macrophage infiltration. Moreover, treatment with phorbol ester resulted in enhanced expression of the Cap43 gene in human monocytic cells in vitro. The expression of the Cap43 gene in infiltrating macrophages is discussed in association with the differentiated or activated status of monocyte/macrophage.",
author = "Akihiro Nishie and Katsuaki Masuda and Michihiro Otsubo and Toshiro Migita and Masazumi Tsuyenosi and Kimitoshi Kohno and Taro Shuin and Seiji Naito and Mayumi Ono and Michihiko Kuwano",
year = "2001",
month = "1",
day = "1",
language = "English",
volume = "7",
pages = "2145--2151",
journal = "Clinical Cancer Research",
issn = "1078-0432",
publisher = "American Association for Cancer Research Inc.",
number = "7",

}

TY - JOUR

T1 - High expression of the Cap43 gene in infiltrating macrophages of human renal cell carcinomas

AU - Nishie, Akihiro

AU - Masuda, Katsuaki

AU - Otsubo, Michihiro

AU - Migita, Toshiro

AU - Tsuyenosi, Masazumi

AU - Kohno, Kimitoshi

AU - Shuin, Taro

AU - Naito, Seiji

AU - Ono, Mayumi

AU - Kuwano, Michihiko

PY - 2001/1/1

Y1 - 2001/1/1

N2 - We used suppression subtractive hybridization to identify highly expressed genes in the cancerous region of human renal cell carcinoma (RCC) compared with noncancerous tissue. Nine genes were identified to show increased expression in the cancerous region compared with the noncancerous region. The nine genes included thymosin β4, secreted protein acidic and rich in cysteine (SPARC), Cap43, ceruloplasmin, serum amyloid A, osteopontin, heat shock protein 90 (HSP90), LOT1, and casein kinase I. Of these 9 genes, in situ hybridization with 10 clinical samples consistently showed a strong expression of Cap43 mRNA in infiltrating macrophages in RCCs, but not in cancer cells proliferating in an alveolar pattern. However, Cap43 mRNA was also apparently detected in epithelial cells of the renal proximal tubuli in noncancerous tissue. The higher expression of the Cap43 gene in the cancerous region of RCCs appears to depend on macrophage infiltration. Moreover, treatment with phorbol ester resulted in enhanced expression of the Cap43 gene in human monocytic cells in vitro. The expression of the Cap43 gene in infiltrating macrophages is discussed in association with the differentiated or activated status of monocyte/macrophage.

AB - We used suppression subtractive hybridization to identify highly expressed genes in the cancerous region of human renal cell carcinoma (RCC) compared with noncancerous tissue. Nine genes were identified to show increased expression in the cancerous region compared with the noncancerous region. The nine genes included thymosin β4, secreted protein acidic and rich in cysteine (SPARC), Cap43, ceruloplasmin, serum amyloid A, osteopontin, heat shock protein 90 (HSP90), LOT1, and casein kinase I. Of these 9 genes, in situ hybridization with 10 clinical samples consistently showed a strong expression of Cap43 mRNA in infiltrating macrophages in RCCs, but not in cancer cells proliferating in an alveolar pattern. However, Cap43 mRNA was also apparently detected in epithelial cells of the renal proximal tubuli in noncancerous tissue. The higher expression of the Cap43 gene in the cancerous region of RCCs appears to depend on macrophage infiltration. Moreover, treatment with phorbol ester resulted in enhanced expression of the Cap43 gene in human monocytic cells in vitro. The expression of the Cap43 gene in infiltrating macrophages is discussed in association with the differentiated or activated status of monocyte/macrophage.

UR - http://www.scopus.com/inward/record.url?scp=0034772253&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034772253&partnerID=8YFLogxK

M3 - Article

C2 - 11448934

AN - SCOPUS:0034772253

VL - 7

SP - 2145

EP - 2151

JO - Clinical Cancer Research

JF - Clinical Cancer Research

SN - 1078-0432

IS - 7

ER -