Abstract
The cytoskeleton is a network of crosslinked, semiflexible filaments, and it has been suggested that it has properties of a glassy state. Here we employ optical-trap-based microrheology to apply forces to a model cytoskeleton and measure the high-bandwidth response at an anterior point. Simulating the highly nonlinear and anisotropic stress-strain propagation assuming affinity, we found that theoretical predictions for the quasistatic response of semiflexible polymers are only realized at high frequencies inaccessible to conventional rheometers. We give a theoretical basis for determining the frequency when both affinity and quasistaticity are valid, and we discuss with experimental evidence that the relaxations at lower frequencies can be characterized by the experimentally obtained nonaffinity parameter.
| Original language | English |
|---|---|
| Article number | 042711 |
| Journal | Physical Review E - Statistical, Nonlinear, and Soft Matter Physics |
| Volume | 89 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Apr 21 2014 |
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All Science Journal Classification (ASJC) codes
- Statistical and Nonlinear Physics
- Statistics and Probability
- Condensed Matter Physics
Cite this
High-frequency affine mechanics and nonaffine relaxation in a model cytoskeleton. / Head, David A.; Ikebe, Emi; Nakamasu, Akiko; Zhang, Peijuan; Villaruz, Lara Gay; Kinoshita, Suguru; Ando, Shoji; Mizuno, Daisuke.
In: Physical Review E - Statistical, Nonlinear, and Soft Matter Physics, Vol. 89, No. 4, 042711, 21.04.2014.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - High-frequency affine mechanics and nonaffine relaxation in a model cytoskeleton
AU - Head, David A.
AU - Ikebe, Emi
AU - Nakamasu, Akiko
AU - Zhang, Peijuan
AU - Villaruz, Lara Gay
AU - Kinoshita, Suguru
AU - Ando, Shoji
AU - Mizuno, Daisuke
PY - 2014/4/21
Y1 - 2014/4/21
N2 - The cytoskeleton is a network of crosslinked, semiflexible filaments, and it has been suggested that it has properties of a glassy state. Here we employ optical-trap-based microrheology to apply forces to a model cytoskeleton and measure the high-bandwidth response at an anterior point. Simulating the highly nonlinear and anisotropic stress-strain propagation assuming affinity, we found that theoretical predictions for the quasistatic response of semiflexible polymers are only realized at high frequencies inaccessible to conventional rheometers. We give a theoretical basis for determining the frequency when both affinity and quasistaticity are valid, and we discuss with experimental evidence that the relaxations at lower frequencies can be characterized by the experimentally obtained nonaffinity parameter.
AB - The cytoskeleton is a network of crosslinked, semiflexible filaments, and it has been suggested that it has properties of a glassy state. Here we employ optical-trap-based microrheology to apply forces to a model cytoskeleton and measure the high-bandwidth response at an anterior point. Simulating the highly nonlinear and anisotropic stress-strain propagation assuming affinity, we found that theoretical predictions for the quasistatic response of semiflexible polymers are only realized at high frequencies inaccessible to conventional rheometers. We give a theoretical basis for determining the frequency when both affinity and quasistaticity are valid, and we discuss with experimental evidence that the relaxations at lower frequencies can be characterized by the experimentally obtained nonaffinity parameter.
UR - http://www.scopus.com/inward/record.url?scp=84899750316&partnerID=8YFLogxK
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U2 - 10.1103/PhysRevE.89.042711
DO - 10.1103/PhysRevE.89.042711
M3 - Article
C2 - 24827282
AN - SCOPUS:84899750316
VL - 89
JO - Physical Review E
JF - Physical Review E
SN - 2470-0045
IS - 4
M1 - 042711
ER -