High-level expression of single-chain Fv-Fc fusion protein in serum and egg white of genetically manipulated chickens by using a retroviral vector

Masamichi Kamihira, Ken Ichiro Ono, Kazuhisa Esaka, Ken Ichi Nishijima, Ryoko Kigaku, Hiroyuki Komatsu, Takashi Yamashita, Kenji Kyogoku, Shinji Iijima

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

We report here the generation of transgenic chickens using a retroviral vector for the production of recombinant proteins. It was found that the transgene expression was suppressed when a Moloney murine leukemia virus-based retroviral vector was injected into chicken embryos at the blastodermal stage. When a concentrated viral solution was injected into the heart of developing embryos after 50 to 60 h of incubation, transgene expression was observed throughout the embryo, including the gonads. For practical production, a retroviral vector encoding an expression cassette of antiprion single-chain Fv fused with the Fc region of human immunoglobulin G1 (scFv-Fc) was injected into chicken embryos. The birds that hatched stably produced scFv-Fc in their serum and eggs at high levels (∼5.6 mg/ml). We obtained transgenic progeny from a transgenic chicken generated with this procedure. The transgene was stably integrated into the chromosomes of transgenic progeny. The transgenic progeny also expressed scFv-Fc in the serum and eggs.

Original languageEnglish
Pages (from-to)10864-10874
Number of pages11
JournalJournal of virology
Volume79
Issue number17
DOIs
Publication statusPublished - Sep 1 2005

Fingerprint

retroviral vectors
Single-Chain Antibodies
Egg White
egg albumen
blood proteins
Blood Proteins
Chickens
Embryonic Structures
Transgenes
genetically modified organisms
chickens
embryo (animal)
transgenes
Eggs
Moloney murine leukemia virus
Murine leukemia virus
Gonads
Serum
Recombinant Proteins
Birds

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

High-level expression of single-chain Fv-Fc fusion protein in serum and egg white of genetically manipulated chickens by using a retroviral vector. / Kamihira, Masamichi; Ono, Ken Ichiro; Esaka, Kazuhisa; Nishijima, Ken Ichi; Kigaku, Ryoko; Komatsu, Hiroyuki; Yamashita, Takashi; Kyogoku, Kenji; Iijima, Shinji.

In: Journal of virology, Vol. 79, No. 17, 01.09.2005, p. 10864-10874.

Research output: Contribution to journalArticle

Kamihira, Masamichi ; Ono, Ken Ichiro ; Esaka, Kazuhisa ; Nishijima, Ken Ichi ; Kigaku, Ryoko ; Komatsu, Hiroyuki ; Yamashita, Takashi ; Kyogoku, Kenji ; Iijima, Shinji. / High-level expression of single-chain Fv-Fc fusion protein in serum and egg white of genetically manipulated chickens by using a retroviral vector. In: Journal of virology. 2005 ; Vol. 79, No. 17. pp. 10864-10874.
@article{78d0fe14b38047d1924329d901acc993,
title = "High-level expression of single-chain Fv-Fc fusion protein in serum and egg white of genetically manipulated chickens by using a retroviral vector",
abstract = "We report here the generation of transgenic chickens using a retroviral vector for the production of recombinant proteins. It was found that the transgene expression was suppressed when a Moloney murine leukemia virus-based retroviral vector was injected into chicken embryos at the blastodermal stage. When a concentrated viral solution was injected into the heart of developing embryos after 50 to 60 h of incubation, transgene expression was observed throughout the embryo, including the gonads. For practical production, a retroviral vector encoding an expression cassette of antiprion single-chain Fv fused with the Fc region of human immunoglobulin G1 (scFv-Fc) was injected into chicken embryos. The birds that hatched stably produced scFv-Fc in their serum and eggs at high levels (∼5.6 mg/ml). We obtained transgenic progeny from a transgenic chicken generated with this procedure. The transgene was stably integrated into the chromosomes of transgenic progeny. The transgenic progeny also expressed scFv-Fc in the serum and eggs.",
author = "Masamichi Kamihira and Ono, {Ken Ichiro} and Kazuhisa Esaka and Nishijima, {Ken Ichi} and Ryoko Kigaku and Hiroyuki Komatsu and Takashi Yamashita and Kenji Kyogoku and Shinji Iijima",
year = "2005",
month = "9",
day = "1",
doi = "10.1128/JVI.79.17.10864-10874.2005",
language = "English",
volume = "79",
pages = "10864--10874",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "17",

}

TY - JOUR

T1 - High-level expression of single-chain Fv-Fc fusion protein in serum and egg white of genetically manipulated chickens by using a retroviral vector

AU - Kamihira, Masamichi

AU - Ono, Ken Ichiro

AU - Esaka, Kazuhisa

AU - Nishijima, Ken Ichi

AU - Kigaku, Ryoko

AU - Komatsu, Hiroyuki

AU - Yamashita, Takashi

AU - Kyogoku, Kenji

AU - Iijima, Shinji

PY - 2005/9/1

Y1 - 2005/9/1

N2 - We report here the generation of transgenic chickens using a retroviral vector for the production of recombinant proteins. It was found that the transgene expression was suppressed when a Moloney murine leukemia virus-based retroviral vector was injected into chicken embryos at the blastodermal stage. When a concentrated viral solution was injected into the heart of developing embryos after 50 to 60 h of incubation, transgene expression was observed throughout the embryo, including the gonads. For practical production, a retroviral vector encoding an expression cassette of antiprion single-chain Fv fused with the Fc region of human immunoglobulin G1 (scFv-Fc) was injected into chicken embryos. The birds that hatched stably produced scFv-Fc in their serum and eggs at high levels (∼5.6 mg/ml). We obtained transgenic progeny from a transgenic chicken generated with this procedure. The transgene was stably integrated into the chromosomes of transgenic progeny. The transgenic progeny also expressed scFv-Fc in the serum and eggs.

AB - We report here the generation of transgenic chickens using a retroviral vector for the production of recombinant proteins. It was found that the transgene expression was suppressed when a Moloney murine leukemia virus-based retroviral vector was injected into chicken embryos at the blastodermal stage. When a concentrated viral solution was injected into the heart of developing embryos after 50 to 60 h of incubation, transgene expression was observed throughout the embryo, including the gonads. For practical production, a retroviral vector encoding an expression cassette of antiprion single-chain Fv fused with the Fc region of human immunoglobulin G1 (scFv-Fc) was injected into chicken embryos. The birds that hatched stably produced scFv-Fc in their serum and eggs at high levels (∼5.6 mg/ml). We obtained transgenic progeny from a transgenic chicken generated with this procedure. The transgene was stably integrated into the chromosomes of transgenic progeny. The transgenic progeny also expressed scFv-Fc in the serum and eggs.

UR - http://www.scopus.com/inward/record.url?scp=23844504527&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=23844504527&partnerID=8YFLogxK

U2 - 10.1128/JVI.79.17.10864-10874.2005

DO - 10.1128/JVI.79.17.10864-10874.2005

M3 - Article

C2 - 16103139

AN - SCOPUS:23844504527

VL - 79

SP - 10864

EP - 10874

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 17

ER -