High-level expression of single-chain Fv-Fc fusion protein in serum and egg white of genetically manipulated chickens by using a retroviral vector

Masamichi Kamihira, Ken Ichiro Ono, Kazuhisa Esaka, Ken Ichi Nishijima, Ryoko Kigaku, Hiroyuki Komatsu, Takashi Yamashita, Kenji Kyogoku, Shinji Iijima

Research output: Contribution to journalArticlepeer-review

90 Citations (Scopus)

Abstract

We report here the generation of transgenic chickens using a retroviral vector for the production of recombinant proteins. It was found that the transgene expression was suppressed when a Moloney murine leukemia virus-based retroviral vector was injected into chicken embryos at the blastodermal stage. When a concentrated viral solution was injected into the heart of developing embryos after 50 to 60 h of incubation, transgene expression was observed throughout the embryo, including the gonads. For practical production, a retroviral vector encoding an expression cassette of antiprion single-chain Fv fused with the Fc region of human immunoglobulin G1 (scFv-Fc) was injected into chicken embryos. The birds that hatched stably produced scFv-Fc in their serum and eggs at high levels (∼5.6 mg/ml). We obtained transgenic progeny from a transgenic chicken generated with this procedure. The transgene was stably integrated into the chromosomes of transgenic progeny. The transgenic progeny also expressed scFv-Fc in the serum and eggs.

Original languageEnglish
Pages (from-to)10864-10874
Number of pages11
JournalJournal of virology
Volume79
Issue number17
DOIs
Publication statusPublished - Sep 2005

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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