High metabolic function of primary human and porcine hepatocytes in a polyurethane foam/spheroid culture system in plasma from patients with fulminant hepatic failure

Y. i. Yamashita, M. Shimada, E. Tsujita, K. Shirabe, H. Ijima, K. Nakazawa, R. Sakiyama, J. Fukuda, K. Funatsu, K. Sugimachi

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

It has been demonstrated that plasma from patients with fulminant hepatic failure (FHF) interferes extensively with cellular function. We placed primary human and primary porcine hepatocytes in a polyurethane foam (PUF)/spheroid culture system and compared the metabolic functions in the plasma of patients with FHF in a 24-h stationary culture to those in a monolayer culture. The PUF/spheroid culture system using primary human and primary porcine hepatocytes significantly decreased ammonia content during 28-day culture. Fisher's ratio significantly increased at culture days 3 and 7. Tauroursodeoxycholic acid significantly increased and glycochenodeoxycholic acid and taurochenodeoxycholic acid decreased in the FHF patients' plasma at culture day 3. During at least a 24-h culture in the FHF patients' plasma, metabolic functions of primary human and primary porcine hepatocytes were almost identical. The present results indicate that the PUF/spheroid culture system using primary human or primary porcine hepatocytes demonstrated more advantageous metabolic functions in the plasma from patients with FHF than the monolayer culture.

Original languageEnglish
Pages (from-to)379-384
Number of pages6
JournalCell Transplantation
Volume11
Issue number4
Publication statusPublished - Aug 19 2002

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Cell Biology
  • Transplantation

Fingerprint Dive into the research topics of 'High metabolic function of primary human and porcine hepatocytes in a polyurethane foam/spheroid culture system in plasma from patients with fulminant hepatic failure'. Together they form a unique fingerprint.

  • Cite this

    Yamashita, Y. I., Shimada, M., Tsujita, E., Shirabe, K., Ijima, H., Nakazawa, K., Sakiyama, R., Fukuda, J., Funatsu, K., & Sugimachi, K. (2002). High metabolic function of primary human and porcine hepatocytes in a polyurethane foam/spheroid culture system in plasma from patients with fulminant hepatic failure. Cell Transplantation, 11(4), 379-384.