High pressure induces G2 arrest in murine erythroleukemia cells

Masaki Matsumoto, Takeo Yamaguchi, Yasuyuki Fukumaki, Reiko Yasunaga, Shigeyuki Terada

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

The effect of high pressure on proliferation and cell cycle progression was examined using murine erythroleukemia (MEL) cells. The MEL cells were exposed to high pressures (0.1-130 MPa) and then cultured for 5 days at atmospheric pressure. The proliferation of MEL cells was unaffected up to 60 MPa, but was suppressed at 80-110 MPa. Above 120 MPa, the cells were fragmented. The cell cycle analysis of 80 MPa-treated MEL cells showed that the cells in S phase are most sensitive to high pressure and they arrest in G2 phase. Interestingly, G2-arrested cells reinitiated DNA synthesis, resulting in giant cells with high DNA contents. Furthermore, when such G2-arrested cells were exposed to caffeine, premature mitosis, characterized by chromosome pulverization, was observed. These results suggest that the suppression of proliferation in high-pressure-treated MEL cells is associated with G2 arrest following S phase delay. Thus, it seems valuable to apply high pressure to the investigation of the cell cycle.

Original languageEnglish
Pages (from-to)87-93
Number of pages7
JournalJournal of Biochemistry
Volume123
Issue number1
DOIs
Publication statusPublished - Jan 1 1998
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Fingerprint Dive into the research topics of 'High pressure induces G2 arrest in murine erythroleukemia cells'. Together they form a unique fingerprint.

Cite this