High production of acetone-butanol-ethanol with high cell density culture by cell-recycling and bleeding

Yukihiro Tashiro, Katsuhisa Takeda, Genta Kobayashi, Kenji Sonomoto

Research output: Contribution to journalArticle

104 Citations (Scopus)

Abstract

A continuous acetone-butanol-ethanol (ABE) production system with high cell density obtained by cell-recycling of Clostridium saccharoperbutylacetonicum N1-4 has been studied. In conventional continuous culture of ABE without cell-recycling, the cell concentration was below 5.2 g l-1 and the maximum ABE productivity was only 1.85 g l-1 h-1 at a dilution rate of 0.20 h-1. To obtain a high cell density at a faster rate, we concentrated the solventogenic cells of the broth 10 times by membrane filtration and were able to obtain approximately 20 g l-1 of active cells after only 12 h of cultivation. Continuous culture with cell-recycling was then started, and the cell concentration increased gradually through cultivation to a value greater than 100 g l-1. The maximum ABE productivity of 11.0 g l-1 h-1 was obtained at a dilution rate of 0.85 h-1. However, a cell concentration greater than 100 g l-1 resulted in heavy bubbling and broth outflow, which made it impossible to carry out continuous culture. Therefore, to maintain a stable cell concentration, cell-bleeding was performed together with cell-recycling. At dilution rates of 0.11 h-1 and above for cell-bleeding, continuous culture with cell-recycling could be operated for more than 200 h without strain degeneration and the overall volumetric ABE productivity of 7.55 g l -1 h-1 was achieved at an ABE concentration of 8.58 g l-1.

Original languageEnglish
Pages (from-to)197-206
Number of pages10
JournalJournal of Biotechnology
Volume120
Issue number2
DOIs
Publication statusPublished - Nov 4 2005

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Butanols
Recycling
Acetone
Butenes
Ethanol
Cell Culture Techniques
Cell Count
Hemorrhage
Dilution
Productivity
Clostridium
Membranes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

High production of acetone-butanol-ethanol with high cell density culture by cell-recycling and bleeding. / Tashiro, Yukihiro; Takeda, Katsuhisa; Kobayashi, Genta; Sonomoto, Kenji.

In: Journal of Biotechnology, Vol. 120, No. 2, 04.11.2005, p. 197-206.

Research output: Contribution to journalArticle

Tashiro, Yukihiro ; Takeda, Katsuhisa ; Kobayashi, Genta ; Sonomoto, Kenji. / High production of acetone-butanol-ethanol with high cell density culture by cell-recycling and bleeding. In: Journal of Biotechnology. 2005 ; Vol. 120, No. 2. pp. 197-206.
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