High-resolution analysis of polyprenols by supercritical fluid chromatography

T. Bamba, E. Fukusaki, S. Kajiyama, K. Ute, T. Kitayama, A. Kobayashi

Research output: Contribution to journalArticlepeer-review

30 Citations (Scopus)


A high-resolution analysis of polyprenol mixtures was achieved by supercritical fluid chromatography (SFC). The separation of polyprenols was examined on an octadecylsilane-packed column with liquid carbon dioxide as the mobile phase and ethanol as modifier. Using this chromatography system, the resolution of separation (Rs) between octadecaprenol (prenol 18) and nonadecaprenol (prenol 19) was two times higher than that using conventional reversed-phase high-performance liquid chromatography. Our SFC technique allows the advantage of baseline separation of polyprenol samples containing hydrophobic components such as terpenes or fatty acids that are unfavorable for good separation. This method is very useful for the analysis of structurally close polyprenol analogues of rubber plant metabolites.

Original languageEnglish
Pages (from-to)113-117
Number of pages5
JournalJournal of Chromatography A
Issue number1
Publication statusPublished - Mar 9 2001
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry


Dive into the research topics of 'High-resolution analysis of polyprenols by supercritical fluid chromatography'. Together they form a unique fingerprint.

Cite this