High-resolution imaging of a cell-attached nanointerface using a gold-nanoparticle two-dimensional sheet

Shihomi Masuda, Yuhki Yanase, Eiji Usukura, Soh Ryuzaki, Pangpang Wang, Koichi Okamoto, Thasaneeya Kuboki, Satoru Kidoaki, Kaoru Tamada

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

This paper proposes a simple, effective, non-scanning method for the visualization of a cell-attached nanointerface. The method uses localized surface plasmon resonance (LSPR) excited homogeneously on a two-dimensional (2D) self-assembled gold-nanoparticle sheet. The LSPR of the gold-nanoparticle sheet provides high-contrast interfacial images due to the confined light within a region a few tens of nanometers from the particles and the enhancement of fluorescence. Test experiments on rat basophilic leukemia (RBL-2H3) cells with fluorescence-labeled actin filaments revealed high axial and lateral resolution even under a regular epifluorescence microscope, which produced higher quality images than those captured under a total internal reflection fluorescence (TIRF) microscope. This non-scanning-type, high-resolution imaging method will be an effective tool for monitoring interfacial phenomena that exhibit relatively rapid reaction kinetics in various cellular and molecular dynamics.

Original languageEnglish
Article number3720
JournalScientific reports
Volume7
Issue number1
DOIs
Publication statusPublished - Dec 1 2017

Fingerprint

gold
surface plasmon resonance
fluorescence
nanoparticles
high resolution
cells
microscopes
leukemias
rats
filaments
reaction kinetics
molecular dynamics
augmentation

All Science Journal Classification (ASJC) codes

  • General

Cite this

High-resolution imaging of a cell-attached nanointerface using a gold-nanoparticle two-dimensional sheet. / Masuda, Shihomi; Yanase, Yuhki; Usukura, Eiji; Ryuzaki, Soh; Wang, Pangpang; Okamoto, Koichi; Kuboki, Thasaneeya; Kidoaki, Satoru; Tamada, Kaoru.

In: Scientific reports, Vol. 7, No. 1, 3720, 01.12.2017.

Research output: Contribution to journalArticle

@article{321e48e53be5405dba7604342b265eea,
title = "High-resolution imaging of a cell-attached nanointerface using a gold-nanoparticle two-dimensional sheet",
abstract = "This paper proposes a simple, effective, non-scanning method for the visualization of a cell-attached nanointerface. The method uses localized surface plasmon resonance (LSPR) excited homogeneously on a two-dimensional (2D) self-assembled gold-nanoparticle sheet. The LSPR of the gold-nanoparticle sheet provides high-contrast interfacial images due to the confined light within a region a few tens of nanometers from the particles and the enhancement of fluorescence. Test experiments on rat basophilic leukemia (RBL-2H3) cells with fluorescence-labeled actin filaments revealed high axial and lateral resolution even under a regular epifluorescence microscope, which produced higher quality images than those captured under a total internal reflection fluorescence (TIRF) microscope. This non-scanning-type, high-resolution imaging method will be an effective tool for monitoring interfacial phenomena that exhibit relatively rapid reaction kinetics in various cellular and molecular dynamics.",
author = "Shihomi Masuda and Yuhki Yanase and Eiji Usukura and Soh Ryuzaki and Pangpang Wang and Koichi Okamoto and Thasaneeya Kuboki and Satoru Kidoaki and Kaoru Tamada",
year = "2017",
month = "12",
day = "1",
doi = "10.1038/s41598-017-04000-4",
language = "English",
volume = "7",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",

}

TY - JOUR

T1 - High-resolution imaging of a cell-attached nanointerface using a gold-nanoparticle two-dimensional sheet

AU - Masuda, Shihomi

AU - Yanase, Yuhki

AU - Usukura, Eiji

AU - Ryuzaki, Soh

AU - Wang, Pangpang

AU - Okamoto, Koichi

AU - Kuboki, Thasaneeya

AU - Kidoaki, Satoru

AU - Tamada, Kaoru

PY - 2017/12/1

Y1 - 2017/12/1

N2 - This paper proposes a simple, effective, non-scanning method for the visualization of a cell-attached nanointerface. The method uses localized surface plasmon resonance (LSPR) excited homogeneously on a two-dimensional (2D) self-assembled gold-nanoparticle sheet. The LSPR of the gold-nanoparticle sheet provides high-contrast interfacial images due to the confined light within a region a few tens of nanometers from the particles and the enhancement of fluorescence. Test experiments on rat basophilic leukemia (RBL-2H3) cells with fluorescence-labeled actin filaments revealed high axial and lateral resolution even under a regular epifluorescence microscope, which produced higher quality images than those captured under a total internal reflection fluorescence (TIRF) microscope. This non-scanning-type, high-resolution imaging method will be an effective tool for monitoring interfacial phenomena that exhibit relatively rapid reaction kinetics in various cellular and molecular dynamics.

AB - This paper proposes a simple, effective, non-scanning method for the visualization of a cell-attached nanointerface. The method uses localized surface plasmon resonance (LSPR) excited homogeneously on a two-dimensional (2D) self-assembled gold-nanoparticle sheet. The LSPR of the gold-nanoparticle sheet provides high-contrast interfacial images due to the confined light within a region a few tens of nanometers from the particles and the enhancement of fluorescence. Test experiments on rat basophilic leukemia (RBL-2H3) cells with fluorescence-labeled actin filaments revealed high axial and lateral resolution even under a regular epifluorescence microscope, which produced higher quality images than those captured under a total internal reflection fluorescence (TIRF) microscope. This non-scanning-type, high-resolution imaging method will be an effective tool for monitoring interfacial phenomena that exhibit relatively rapid reaction kinetics in various cellular and molecular dynamics.

UR - http://www.scopus.com/inward/record.url?scp=85020929452&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85020929452&partnerID=8YFLogxK

U2 - 10.1038/s41598-017-04000-4

DO - 10.1038/s41598-017-04000-4

M3 - Article

C2 - 28623338

AN - SCOPUS:85020929452

VL - 7

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

IS - 1

M1 - 3720

ER -