High-resolution mapping of grh6, a gene from oryza nivara (Sharma et shastry) conferring resistance to green rice leafhopper (nephotettix cincticeps uhler)

Cong Nguyen Phi, Daisuke Fujita, Yoshiyuki Yamagata, Atsushi Yoshimura, Hideshi Yasui

Research output: Contribution to journalArticle

Abstract

The green rice leafhopper (GRH), Nephotettix cincticeps Uhler, is a major insect pest of cultivated rice, Oryza sativa L., throughout the temperate regions of East Asia. GRH resistance had been reported in the wild species Oryza nivara but genetic basis of GRH resistance in wild rice accession has not been clarified. Here, we found a major QTL, qGRH4.2, on chromosome 4 conferred GRH resistance with 14.1 of the logarithm of odds (LOD) score explaining 67.6% of phenotypic variance in the BC1F1 population derived from a cross between the susceptible japonica cultivar ‘Taichung 65’ (T65) and O. nivara accession IRGC105715. qGRH4.2 has been identified as GRH6 between the markers RM5414 and C60248 in a BC3F2 population derived from two BC3F1 plants resistant to GRH. In a high-resolution mapping, the GRH6 region was delimited between the markers G6-c60k and 7L16f, and corresponded to an 31.2-kbp region of the ‘Nipponbare’ genome. Understanding the genetic basis of GRH resistance will facilitate the use of GRH resistance genes in marker-assisted breeding in rice.

Original languageEnglish
Pages (from-to)439-446
Number of pages8
JournalBreeding Science
Volume69
Issue number3
DOIs
Publication statusPublished - Jan 1 2019

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Oryza nivara
Nephotettix cincticeps
Nephotettix nigropictus
Hemiptera
chromosome mapping
Genes
rice
wild rice
Oryza
East Asia
phenotypic variation
insect pests
quantitative trait loci
Oryza sativa
Chromosomes, Human, Pair 4
Far East
chromosomes
genetic markers
genome
breeding

All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

Cite this

High-resolution mapping of grh6, a gene from oryza nivara (Sharma et shastry) conferring resistance to green rice leafhopper (nephotettix cincticeps uhler). / Phi, Cong Nguyen; Fujita, Daisuke; Yamagata, Yoshiyuki; Yoshimura, Atsushi; Yasui, Hideshi.

In: Breeding Science, Vol. 69, No. 3, 01.01.2019, p. 439-446.

Research output: Contribution to journalArticle

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