High-resolution spatial and temporal analysis of phytoalexin production in oats

Yoshihiro Izumi, Shin'Ichiro Kajiyama, Ryosuke Nakamura, Atsushi Ishihara, Atsushi Okazawa, Eiichiro Fukusaki, Yasuo Kanematsu, Akio Kobayashi

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The production of oat (Avena sativa L.) phytoalexins, avenanthramides, occurs in response to elicitor treatment with oligo-N- acetylchitooligosaccharides. In this study, avenanthramides production was investigated by techniques that provide high spatial and temporal resolution in order to clarify the process of phytoalexin production at the cellular level. The amount of avenanthramides accumulation in a single mesophyll cell was quantified by a combination of laser micro-sampling and low-diffuse nanoflow liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) techniques. Avenanthramides, NAD(P)H and chlorophyll were also visualized in elicitor-treated mesophyll cells using line-scanning fluorescence microscopy. We found that elicitor-treated mesophyll cells could be categorized into three characteristic cell phases, which occurred serially over time. Phase 0 indicated the normal cell state before metabolic or morphological change in response to elicitor, in which the cells contained abundant NAD(P)H. In phase 1, rapid NAD(P)H oxidation and marked movement of chloroplasts occurred, and this phase was the early stage of avenanthramides biosynthesis. In phase 2, avenanthramides accumulation was maximized, and chloroplasts were degraded. Avenanthramides appear to be synthesized in the chloroplast, because a fluorescence signal originating from avenanthramides was localized to the chloroplasts. Moreover, our results indicated that avenanthramides biosynthesis and the hypersensitive response (HR) occurred in identical cells. Thus, the avenanthramides production may be one of sequential events programmed in HR leading to cell death. Furthermore, the phase of the defense response was different among mesophyll cells simultaneously treated with elicitor. These results suggest that individual cells may have different susceptibility to the elicitor.

Original languageEnglish
Pages (from-to)931-943
Number of pages13
JournalPlanta
Volume229
Issue number4
DOIs
Publication statusPublished - Mar 1 2009
Externally publishedYes

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Spatio-Temporal Analysis
phytoalexins
oats
Mesophyll Cells
Chloroplasts
mesophyll
chloroplasts
NAD
cells
hypersensitive response
avenanthramides
Avena
avenanthramide-2C
biosynthesis
Electrospray Ionization Mass Spectrometry
Avena sativa
Chlorophyll
Tandem Mass Spectrometry
fluorescence microscopy
Fluorescence Microscopy

All Science Journal Classification (ASJC) codes

  • Genetics
  • Plant Science

Cite this

Izumi, Y., Kajiyama, SI., Nakamura, R., Ishihara, A., Okazawa, A., Fukusaki, E., ... Kobayashi, A. (2009). High-resolution spatial and temporal analysis of phytoalexin production in oats. Planta, 229(4), 931-943. https://doi.org/10.1007/s00425-008-0887-x

High-resolution spatial and temporal analysis of phytoalexin production in oats. / Izumi, Yoshihiro; Kajiyama, Shin'Ichiro; Nakamura, Ryosuke; Ishihara, Atsushi; Okazawa, Atsushi; Fukusaki, Eiichiro; Kanematsu, Yasuo; Kobayashi, Akio.

In: Planta, Vol. 229, No. 4, 01.03.2009, p. 931-943.

Research output: Contribution to journalArticle

Izumi, Y, Kajiyama, SI, Nakamura, R, Ishihara, A, Okazawa, A, Fukusaki, E, Kanematsu, Y & Kobayashi, A 2009, 'High-resolution spatial and temporal analysis of phytoalexin production in oats', Planta, vol. 229, no. 4, pp. 931-943. https://doi.org/10.1007/s00425-008-0887-x
Izumi Y, Kajiyama SI, Nakamura R, Ishihara A, Okazawa A, Fukusaki E et al. High-resolution spatial and temporal analysis of phytoalexin production in oats. Planta. 2009 Mar 1;229(4):931-943. https://doi.org/10.1007/s00425-008-0887-x
Izumi, Yoshihiro ; Kajiyama, Shin'Ichiro ; Nakamura, Ryosuke ; Ishihara, Atsushi ; Okazawa, Atsushi ; Fukusaki, Eiichiro ; Kanematsu, Yasuo ; Kobayashi, Akio. / High-resolution spatial and temporal analysis of phytoalexin production in oats. In: Planta. 2009 ; Vol. 229, No. 4. pp. 931-943.
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