Abstract
This work presents high sensitive detection of specific adsorption of proteins onto a silicon oxide (SiOx) surface modified by molecules with a biotinyl-end group. We fabricated a SiOx waveguide by sputtering onto a thin gold film and measured the incident angle-dependence of reflectivity using a polarized He-Ne laser under total internal reflection condition. When the s-polarized light was irradiated to the SiOx waveguide (760 nm) coupled with a prism, the incident angle-dependence of reflectivity showed narrow dips due to excitation of the waveguide mode. The waveguide surface was modified by the molecules with the biotinyl-end group as a protein probe. Specific adsorption of streptavidin in a PBS buffer solution was monitored through time course of the reflectivity change at a constant incident angle, where the waveguide mode was excited. As compared with conventional SPR system using a gold surface, high sensitive detection of the specific adsorption was achieved. Additionally, we fabricated micro-patterned arrays of biotinyl-end layers onto the SiOx waveguide surface by micro-contact printing (μCP). Then we observed the specific adsorption behavior on the micro-patterned arrays by the imaging based on the waveguide mode using a CCD camera.
Original language | English |
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Title of host publication | Nanoparticles and Nanostructures in Sensors and Catalysis |
Pages | 470-475 |
Number of pages | 6 |
Volume | 900 |
Publication status | Published - Dec 1 2005 |
Externally published | Yes |
Event | 2005 MRS Fall Meeting - Boston, MA, United States Duration: Nov 28 2005 → Dec 2 2005 |
Other
Other | 2005 MRS Fall Meeting |
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Country | United States |
City | Boston, MA |
Period | 11/28/05 → 12/2/05 |
All Science Journal Classification (ASJC) codes
- Materials Science(all)
- Condensed Matter Physics
- Mechanics of Materials
- Mechanical Engineering