High sensitive optical detection of bio-chemicals onto a silicon oxide surface based on waveguide mode

Nobuko Fukuda, Makoto Fujimaki, Koichi Awazu, Kaoru Tamada, Kiyoslii Yase

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

This work presents high sensitive detection of specific adsorption of proteins onto a silicon oxide (SiOx) surface modified by molecules with a biotinyl-end group. We fabricated a SiOx waveguide by sputtering onto a thin gold film and measured the incident angle-dependence of reflectivity using a polarized He-Ne laser under total internal reflection condition. When the s-polarized light was irradiated to the SiOx waveguide (760 nm) coupled with a prism, the incident angle-dependence of reflectivity showed narrow dips due to excitation of the waveguide mode. The waveguide surface was modified by the molecules with the biotinyl-end group as a protein probe. Specific adsorption of streptavidin in a PBS buffer solution was monitored through time course of the reflectivity change at a constant incident angle, where the waveguide mode was excited. As compared with conventional SPR system using a gold surface, high sensitive detection of the specific adsorption was achieved. Additionally, we fabricated micro-patterned arrays of biotinyl-end layers onto the SiOx waveguide surface by micro-contact printing (μCP). Then we observed the specific adsorption behavior on the micro-patterned arrays by the imaging based on the waveguide mode using a CCD camera.

Original languageEnglish
Title of host publicationNanoparticles and Nanostructures in Sensors and Catalysis
Pages470-475
Number of pages6
Volume900
Publication statusPublished - Dec 1 2005
Externally publishedYes
Event2005 MRS Fall Meeting - Boston, MA, United States
Duration: Nov 28 2005Dec 2 2005

Other

Other2005 MRS Fall Meeting
CountryUnited States
CityBoston, MA
Period11/28/0512/2/05

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

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