Abstract
This work presents high sensitive detection of specific adsorption of proteins onto a silicon oxide (SiOx) surface modified by molecules with a biotinyl-end group. We fabricated a SiOx waveguide by sputtering onto a thin gold film and measured the incident angle-dependence of reflectivity using a polarized He-Ne laser under total internal reflection condition. When the s-polarized light was irradiated to the SiOx waveguide (760 nm) coupled with a prism, the incident angle-dependence of reflectivity showed narrow dips due to excitation of the waveguide mode. The waveguide surface was modified by the molecules with the biotinyl-end group as a protein probe. Specific adsorption of streptavidin in a PBS buffer solution was monitored through time course of the reflectivity change at a constant incident angle, where the waveguide mode was excited. As compared with conventional SPR system using a gold surface, high sensitive detection of the specific adsorption was achieved. Additionally, we fabricated micro-patterned arrays of biotinyl-end layers onto the SiOx waveguide surface by micro-contact printing (μCP). Then we observed the specific adsorption behavior on the micro-patterned arrays by the imaging based on the waveguide mode using a CCD camera.
| Original language | English |
|---|---|
| Title of host publication | Nanoparticles and Nanostructures in Sensors and Catalysis |
| Pages | 470-475 |
| Number of pages | 6 |
| Volume | 900 |
| Publication status | Published - Dec 1 2005 |
| Externally published | Yes |
| Event | 2005 MRS Fall Meeting - Boston, MA, United States Duration: Nov 28 2005 → Dec 2 2005 |
Other
| Other | 2005 MRS Fall Meeting |
|---|---|
| Country | United States |
| City | Boston, MA |
| Period | 11/28/05 → 12/2/05 |
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All Science Journal Classification (ASJC) codes
- Materials Science(all)
- Condensed Matter Physics
- Mechanics of Materials
- Mechanical Engineering
Cite this
High sensitive optical detection of bio-chemicals onto a silicon oxide surface based on waveguide mode. / Fukuda, Nobuko; Fujimaki, Makoto; Awazu, Koichi; Tamada, Kaoru; Yase, Kiyoslii.
Nanoparticles and Nanostructures in Sensors and Catalysis. Vol. 900 2005. p. 470-475.Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - High sensitive optical detection of bio-chemicals onto a silicon oxide surface based on waveguide mode
AU - Fukuda, Nobuko
AU - Fujimaki, Makoto
AU - Awazu, Koichi
AU - Tamada, Kaoru
AU - Yase, Kiyoslii
PY - 2005/12/1
Y1 - 2005/12/1
N2 - This work presents high sensitive detection of specific adsorption of proteins onto a silicon oxide (SiOx) surface modified by molecules with a biotinyl-end group. We fabricated a SiOx waveguide by sputtering onto a thin gold film and measured the incident angle-dependence of reflectivity using a polarized He-Ne laser under total internal reflection condition. When the s-polarized light was irradiated to the SiOx waveguide (760 nm) coupled with a prism, the incident angle-dependence of reflectivity showed narrow dips due to excitation of the waveguide mode. The waveguide surface was modified by the molecules with the biotinyl-end group as a protein probe. Specific adsorption of streptavidin in a PBS buffer solution was monitored through time course of the reflectivity change at a constant incident angle, where the waveguide mode was excited. As compared with conventional SPR system using a gold surface, high sensitive detection of the specific adsorption was achieved. Additionally, we fabricated micro-patterned arrays of biotinyl-end layers onto the SiOx waveguide surface by micro-contact printing (μCP). Then we observed the specific adsorption behavior on the micro-patterned arrays by the imaging based on the waveguide mode using a CCD camera.
AB - This work presents high sensitive detection of specific adsorption of proteins onto a silicon oxide (SiOx) surface modified by molecules with a biotinyl-end group. We fabricated a SiOx waveguide by sputtering onto a thin gold film and measured the incident angle-dependence of reflectivity using a polarized He-Ne laser under total internal reflection condition. When the s-polarized light was irradiated to the SiOx waveguide (760 nm) coupled with a prism, the incident angle-dependence of reflectivity showed narrow dips due to excitation of the waveguide mode. The waveguide surface was modified by the molecules with the biotinyl-end group as a protein probe. Specific adsorption of streptavidin in a PBS buffer solution was monitored through time course of the reflectivity change at a constant incident angle, where the waveguide mode was excited. As compared with conventional SPR system using a gold surface, high sensitive detection of the specific adsorption was achieved. Additionally, we fabricated micro-patterned arrays of biotinyl-end layers onto the SiOx waveguide surface by micro-contact printing (μCP). Then we observed the specific adsorption behavior on the micro-patterned arrays by the imaging based on the waveguide mode using a CCD camera.
UR - http://www.scopus.com/inward/record.url?scp=34249945600&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34249945600&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:34249945600
SN - 1558998543
SN - 9781558998544
VL - 900
SP - 470
EP - 475
BT - Nanoparticles and Nanostructures in Sensors and Catalysis
ER -