High sensitive optical detection of bio-chemicals onto a silicon oxide surface based on waveguide mode

Nobuko Fukuda, Makoto Fujimaki, Koichi Awazu, Kaoru Tamada, Kiyoslii Yase

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

This work presents high sensitive detection of specific adsorption of proteins onto a silicon oxide (SiOx) surface modified by molecules with a biotinyl-end group. We fabricated a SiOx waveguide by sputtering onto a thin gold film and measured the incident angle-dependence of reflectivity using a polarized He-Ne laser under total internal reflection condition. When the s-polarized light was irradiated to the SiOx waveguide (760 nm) coupled with a prism, the incident angle-dependence of reflectivity showed narrow dips due to excitation of the waveguide mode. The waveguide surface was modified by the molecules with the biotinyl-end group as a protein probe. Specific adsorption of streptavidin in a PBS buffer solution was monitored through time course of the reflectivity change at a constant incident angle, where the waveguide mode was excited. As compared with conventional SPR system using a gold surface, high sensitive detection of the specific adsorption was achieved. Additionally, we fabricated micro-patterned arrays of biotinyl-end layers onto the SiOx waveguide surface by micro-contact printing (μCP). Then we observed the specific adsorption behavior on the micro-patterned arrays by the imaging based on the waveguide mode using a CCD camera.

Original languageEnglish
Title of host publicationNanoparticles and Nanostructures in Sensors and Catalysis
Pages470-475
Number of pages6
Volume900
Publication statusPublished - Dec 1 2005
Externally publishedYes
Event2005 MRS Fall Meeting - Boston, MA, United States
Duration: Nov 28 2005Dec 2 2005

Other

Other2005 MRS Fall Meeting
CountryUnited States
CityBoston, MA
Period11/28/0512/2/05

Fingerprint

Silicon oxides
silicon oxides
Waveguides
waveguides
Adsorption
adsorption
reflectance
Gold
gold
proteins
Proteins
Molecules
Streptavidin
Light polarization
CCD cameras
Prisms
printing
polarized light
prisms
Sputtering

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

Cite this

Fukuda, N., Fujimaki, M., Awazu, K., Tamada, K., & Yase, K. (2005). High sensitive optical detection of bio-chemicals onto a silicon oxide surface based on waveguide mode. In Nanoparticles and Nanostructures in Sensors and Catalysis (Vol. 900, pp. 470-475)

High sensitive optical detection of bio-chemicals onto a silicon oxide surface based on waveguide mode. / Fukuda, Nobuko; Fujimaki, Makoto; Awazu, Koichi; Tamada, Kaoru; Yase, Kiyoslii.

Nanoparticles and Nanostructures in Sensors and Catalysis. Vol. 900 2005. p. 470-475.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Fukuda, N, Fujimaki, M, Awazu, K, Tamada, K & Yase, K 2005, High sensitive optical detection of bio-chemicals onto a silicon oxide surface based on waveguide mode. in Nanoparticles and Nanostructures in Sensors and Catalysis. vol. 900, pp. 470-475, 2005 MRS Fall Meeting, Boston, MA, United States, 11/28/05.
Fukuda N, Fujimaki M, Awazu K, Tamada K, Yase K. High sensitive optical detection of bio-chemicals onto a silicon oxide surface based on waveguide mode. In Nanoparticles and Nanostructures in Sensors and Catalysis. Vol. 900. 2005. p. 470-475
Fukuda, Nobuko ; Fujimaki, Makoto ; Awazu, Koichi ; Tamada, Kaoru ; Yase, Kiyoslii. / High sensitive optical detection of bio-chemicals onto a silicon oxide surface based on waveguide mode. Nanoparticles and Nanostructures in Sensors and Catalysis. Vol. 900 2005. pp. 470-475
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