High speed DNA denaturation using microheating devices

Masayuki Furuhashi, Yukihiro Okamoto, Daisuke Onoshima, Takahito Ohshiro, Sou Ryuzaki, Kazumichi Yokota, Makusu Tsutsui, Masateru Taniguchi, Kazuhiko Nakatani, Yoshinobu Baba, Tomoji Kawai

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3 Citations (Scopus)

Abstract

Denaturation is a first step for further treatment of DNA and is expected to be carried out rapidly on an integrated chip. A microheater is a promising device for the denaturation because of easiness for fabrication and operation. In the present study, we fabricated a microheater and thermometers on a coverslip and investigated response of temperature to application of voltage. In addition, our experiment and simulation proved local heating at an aimed area. Finally, we demonstrated denaturation of DNA in buffer solution, the result of which proved that the DNA around the heater denatured within 60 ms.

Original languageEnglish
Article number023112
JournalApplied Physics Letters
Volume103
Issue number2
DOIs
Publication statusPublished - Jul 8 2013
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Physics and Astronomy (miscellaneous)

Cite this

Furuhashi, M., Okamoto, Y., Onoshima, D., Ohshiro, T., Ryuzaki, S., Yokota, K., ... Kawai, T. (2013). High speed DNA denaturation using microheating devices. Applied Physics Letters, 103(2), [023112]. https://doi.org/10.1063/1.4813552