High speed DNA denaturation using microheating devices

Masayuki Furuhashi; Yukihiro Okamoto; Daisuke Onoshima; Takahito Ohshiro; Sou Ryuzaki; Kazumichi Yokota; Makusu Tsutsui; Masateru Taniguchi; Kazuhiko Nakatani; Yoshinobu Baba; Tomoji Kawai

doi:10.1063/1.4813552

High speed DNA denaturation using microheating devices

Masayuki Furuhashi, Yukihiro Okamoto, Daisuke Onoshima, Takahito Ohshiro, Sou Ryuzaki, Kazumichi Yokota, Makusu Tsutsui, Masateru Taniguchi, Kazuhiko Nakatani, Yoshinobu Baba, Tomoji Kawai

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Denaturation is a first step for further treatment of DNA and is expected to be carried out rapidly on an integrated chip. A microheater is a promising device for the denaturation because of easiness for fabrication and operation. In the present study, we fabricated a microheater and thermometers on a coverslip and investigated response of temperature to application of voltage. In addition, our experiment and simulation proved local heating at an aimed area. Finally, we demonstrated denaturation of DNA in buffer solution, the result of which proved that the DNA around the heater denatured within 60 ms.

Original language	English
Article number	023112
Journal	Applied Physics Letters
Volume	103
Issue number	2
DOIs	https://doi.org/10.1063/1.4813552
Publication status	Published - Jul 8 2013
Externally published	Yes

All Science Journal Classification (ASJC) codes

Physics and Astronomy (miscellaneous)

Access to Document

10.1063/1.4813552

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High speed DNA denaturation using microheating devices. / Furuhashi, Masayuki; Okamoto, Yukihiro; Onoshima, Daisuke; Ohshiro, Takahito; Ryuzaki, Sou; Yokota, Kazumichi; Tsutsui, Makusu; Taniguchi, Masateru; Nakatani, Kazuhiko; Baba, Yoshinobu; Kawai, Tomoji.

In: Applied Physics Letters, Vol. 103, No. 2, 023112, 08.07.2013.

Research output: Contribution to journal › Article › peer-review

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AU - Yokota, Kazumichi

AU - Tsutsui, Makusu

AU - Taniguchi, Masateru

AU - Nakatani, Kazuhiko

AU - Baba, Yoshinobu

AU - Kawai, Tomoji

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