High speed DNA denaturation using microheating devices

Masayuki Furuhashi; Yukihiro Okamoto; Daisuke Onoshima; Takahito Ohshiro; Sou Ryuzaki; Kazumichi Yokota; Makusu Tsutsui; Masateru Taniguchi; Kazuhiko Nakatani; Yoshinobu Baba; Tomoji Kawai

doi:10.1063/1.4813552

High speed DNA denaturation using microheating devices

Masayuki Furuhashi, Yukihiro Okamoto, Daisuke Onoshima, Takahito Ohshiro, Sou Ryuzaki, Kazumichi Yokota, Makusu Tsutsui, Masateru Taniguchi, Kazuhiko Nakatani, Yoshinobu Baba, Tomoji Kawai

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Denaturation is a first step for further treatment of DNA and is expected to be carried out rapidly on an integrated chip. A microheater is a promising device for the denaturation because of easiness for fabrication and operation. In the present study, we fabricated a microheater and thermometers on a coverslip and investigated response of temperature to application of voltage. In addition, our experiment and simulation proved local heating at an aimed area. Finally, we demonstrated denaturation of DNA in buffer solution, the result of which proved that the DNA around the heater denatured within 60 ms.

Original language	English
Article number	023112
Journal	Applied Physics Letters
Volume	103
Issue number	2
DOIs	https://doi.org/10.1063/1.4813552
Publication status	Published - Jul 8 2013
Externally published	Yes

All Science Journal Classification (ASJC) codes

Physics and Astronomy (miscellaneous)

Access to Document

10.1063/1.4813552

Cite this

High speed DNA denaturation using microheating devices. / Furuhashi, Masayuki; Okamoto, Yukihiro; Onoshima, Daisuke; Ohshiro, Takahito; Ryuzaki, Sou; Yokota, Kazumichi; Tsutsui, Makusu; Taniguchi, Masateru; Nakatani, Kazuhiko; Baba, Yoshinobu; Kawai, Tomoji.

In: Applied Physics Letters, Vol. 103, No. 2, 023112, 08.07.2013.

Research output: Contribution to journal › Article › peer-review

@article{48e691b15fdd4672a4500fd8e9c13dfc,

title = "High speed DNA denaturation using microheating devices",

abstract = "Denaturation is a first step for further treatment of DNA and is expected to be carried out rapidly on an integrated chip. A microheater is a promising device for the denaturation because of easiness for fabrication and operation. In the present study, we fabricated a microheater and thermometers on a coverslip and investigated response of temperature to application of voltage. In addition, our experiment and simulation proved local heating at an aimed area. Finally, we demonstrated denaturation of DNA in buffer solution, the result of which proved that the DNA around the heater denatured within 60 ms.",

author = "Masayuki Furuhashi and Yukihiro Okamoto and Daisuke Onoshima and Takahito Ohshiro and Sou Ryuzaki and Kazumichi Yokota and Makusu Tsutsui and Masateru Taniguchi and Kazuhiko Nakatani and Yoshinobu Baba and Tomoji Kawai",

note = "Funding Information: This research was supported by the Funding Program for World-Leading Innovative Research and Development on Science and Technology “Innovative NanoBiodevice based on Single Molecule Analysis” from the Japan Society for the Promotion of Science. M.F. acknowledges the support from KAKENHI (24710150). ",

year = "2013",

month = jul,

day = "8",

doi = "10.1063/1.4813552",

language = "English",

volume = "103",

journal = "Applied Physics Letters",

issn = "0003-6951",

publisher = "American Institute of Physics Publising LLC",

number = "2",

}

TY - JOUR

T1 - High speed DNA denaturation using microheating devices

AU - Furuhashi, Masayuki

AU - Okamoto, Yukihiro

AU - Onoshima, Daisuke

AU - Ohshiro, Takahito

AU - Ryuzaki, Sou

AU - Yokota, Kazumichi

AU - Tsutsui, Makusu

AU - Taniguchi, Masateru

AU - Nakatani, Kazuhiko

AU - Baba, Yoshinobu

AU - Kawai, Tomoji

N1 - Funding Information: This research was supported by the Funding Program for World-Leading Innovative Research and Development on Science and Technology “Innovative NanoBiodevice based on Single Molecule Analysis” from the Japan Society for the Promotion of Science. M.F. acknowledges the support from KAKENHI (24710150).

PY - 2013/7/8

Y1 - 2013/7/8

N2 - Denaturation is a first step for further treatment of DNA and is expected to be carried out rapidly on an integrated chip. A microheater is a promising device for the denaturation because of easiness for fabrication and operation. In the present study, we fabricated a microheater and thermometers on a coverslip and investigated response of temperature to application of voltage. In addition, our experiment and simulation proved local heating at an aimed area. Finally, we demonstrated denaturation of DNA in buffer solution, the result of which proved that the DNA around the heater denatured within 60 ms.

AB - Denaturation is a first step for further treatment of DNA and is expected to be carried out rapidly on an integrated chip. A microheater is a promising device for the denaturation because of easiness for fabrication and operation. In the present study, we fabricated a microheater and thermometers on a coverslip and investigated response of temperature to application of voltage. In addition, our experiment and simulation proved local heating at an aimed area. Finally, we demonstrated denaturation of DNA in buffer solution, the result of which proved that the DNA around the heater denatured within 60 ms.

UR - http://www.scopus.com/inward/record.url?scp=84880490357&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84880490357&partnerID=8YFLogxK

U2 - 10.1063/1.4813552

DO - 10.1063/1.4813552

M3 - Article

AN - SCOPUS:84880490357

VL - 103

JO - Applied Physics Letters

JF - Applied Physics Letters

SN - 0003-6951

IS - 2

M1 - 023112

ER -

High speed DNA denaturation using microheating devices

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this