High-throughput and highly sensitive analysis method for polyisoprene in plants by pyrolysis-gas chromatography/mass spectrometry

Shinya Takeno, Takeshi Bamba, Yoshihisa Nakazawa, Eiichiro Fukusaki, Atsushi Okazawa, Akio Kobayashi

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Natural polyisoprene is a biopolymer consisting of isoprene units (C 5H8) that is used commercially in household, medical, and industrial materials. For the management of natural polyisoprene production, the selection of high-yield polyisoprene-producing trees, and an understanding of polyisoprene biosynthesis, a high-throughput and highly sensitive screening method for the quantification of polyisoprene is required. In this study, we examined pyrolysates from polyisoprenes, polyprenols, carotenoids, ubiquinone (CoQ-10), and sterols by pyrolysis gas chromatography/mass spectrometry (PyGC/MS) and determined that the amounts of isoprene and limonene released from polyprenols and polyisoprenes were dependent upon their molecular weights. Based on these results, we developed a relative quantification method for polyisoprene in leaves by direct analysis of 1 mg of leaves using PyGC/MS. This novel quantification method eliminated extraction steps and can be used in the measurement of polyisoprene contents in Eucommia ulmoides and Hevea brasiliensis.

Original languageEnglish
Pages (from-to)13-17
Number of pages5
JournalBioscience, Biotechnology and Biochemistry
Volume74
Issue number1
DOIs
Publication statusPublished - Feb 5 2010

Fingerprint

Polyisoprenes
Gas chromatography
Gas Chromatography-Mass Spectrometry
Mass spectrometry
coenzyme Q10
Pyrolysis
Throughput
Eucommiaceae
Hevea
Biopolymers
Sterols
Carotenoids
Molecular Weight
Ubiquinone
Biosynthesis
isoprene
Screening
Molecular weight

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this

High-throughput and highly sensitive analysis method for polyisoprene in plants by pyrolysis-gas chromatography/mass spectrometry. / Takeno, Shinya; Bamba, Takeshi; Nakazawa, Yoshihisa; Fukusaki, Eiichiro; Okazawa, Atsushi; Kobayashi, Akio.

In: Bioscience, Biotechnology and Biochemistry, Vol. 74, No. 1, 05.02.2010, p. 13-17.

Research output: Contribution to journalArticle

Takeno, Shinya ; Bamba, Takeshi ; Nakazawa, Yoshihisa ; Fukusaki, Eiichiro ; Okazawa, Atsushi ; Kobayashi, Akio. / High-throughput and highly sensitive analysis method for polyisoprene in plants by pyrolysis-gas chromatography/mass spectrometry. In: Bioscience, Biotechnology and Biochemistry. 2010 ; Vol. 74, No. 1. pp. 13-17.
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