High-throughput and sensitive analysis of 3-monochloropropane-1,2-diol fatty acid esters in edible oils by supercritical fluid chromatography/tandem mass spectrometry

Katsuhito Hori, Atsuki Matsubara, Takato Uchikata, Kazunobu Tsumura, Eiichiro Fukusaki, Takeshi Bamba

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

We have established a high-throughput and sensitive analytical method based on supercritical fluid chromatography (SFC) coupled with triple quadrupole mass spectrometry (QqQ MS) for 3-monochloropropane-1,2-diol (3-MCPD) fatty acid esters in edible oils. All analytes were successfully separated within 9. min without sample purification. The system was precise and sensitive, with a limit of detection less than 0.063. mg/kg. The recovery rate of 3-MCPD fatty acid esters spiked into oil samples was in the range of 62.68-115.23%. Furthermore, several edible oils were tested for analyzing 3-MCPD fatty acid ester profiles. This is the first report on the analysis of 3-MCPD fatty acid esters by SFC/QqQ MS. The developed method will be a powerful tool for investigating 3-MCPD fatty acid esters in edible oils.

Original languageEnglish
Pages (from-to)99-104
Number of pages6
JournalJournal of Chromatography A
Volume1250
DOIs
Publication statusPublished - Aug 10 2012
Externally publishedYes

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alpha-Chlorohydrin
Supercritical Fluid Chromatography
Tandem Mass Spectrometry
Oils and fats
Mass spectrometry
Oils
Esters
Fatty Acids
Throughput
Purification
Limit of Detection
Mass Spectrometry
Recovery

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

Cite this

High-throughput and sensitive analysis of 3-monochloropropane-1,2-diol fatty acid esters in edible oils by supercritical fluid chromatography/tandem mass spectrometry. / Hori, Katsuhito; Matsubara, Atsuki; Uchikata, Takato; Tsumura, Kazunobu; Fukusaki, Eiichiro; Bamba, Takeshi.

In: Journal of Chromatography A, Vol. 1250, 10.08.2012, p. 99-104.

Research output: Contribution to journalArticle

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