High-throughput detection of protein kinase activities in cell lysate based on the aggregation of gold nanoparticles with peptides

Yoshiki Katayama, Hirotaro Kitazaki, Jeong Hun Kang, Xiaoming Han, Takeshi Mori, Takuro Niidome

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Cationic peptides were used as coagulant of gold nano-particle (GNP, 20 nm) for the monitoring cellular protein kinase activities. If cationic peptides, which are designed as specific substrates to target protein kinases, were added into the GNP dispersion, the color of the dispersion changed from red to blue due to the aggregation. On the other hand, if the peptide was phosphorylated with target protein kinase, addition of the phosphorylated peptide didn't change the color of the GNP dispersion. Thus, this system can be a rapid assay of protein kinase activity. The method was applicable to the assay of intracellular protein kinase by using cell lysate. In addition, we applied the system for high-throughput screening of protein kinase inhibitor using 1300 kinds of chemical library. The result indicated that the method was also useful for the drug discovery.

Original languageEnglish
Title of host publicationBiological Imaging and Sensing Using Nanoparticle Assemblies
Pages39-44
Number of pages6
Publication statusPublished - Oct 15 2010
Event2009 MRS Fall Meeting - Boston, MA, United States
Duration: Nov 30 2009Dec 4 2009

Publication series

NameMaterials Research Society Symposium Proceedings
Volume1241
ISSN (Print)0272-9172

Other

Other2009 MRS Fall Meeting
CountryUnited States
CityBoston, MA
Period11/30/0912/4/09

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All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

Cite this

Katayama, Y., Kitazaki, H., Kang, J. H., Han, X., Mori, T., & Niidome, T. (2010). High-throughput detection of protein kinase activities in cell lysate based on the aggregation of gold nanoparticles with peptides. In Biological Imaging and Sensing Using Nanoparticle Assemblies (pp. 39-44). (Materials Research Society Symposium Proceedings; Vol. 1241).