High-throughput detection of protein kinase activities in cell lysate based on the aggregation of gold nanoparticles with peptides

Yoshiki Katayama, Hirotaro Kitazaki, Jeong Hun Kang, Xiaoming Han, Takeshi Mori, Takuro Niidome

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Abstract

    Cationic peptides were used as coagulant of gold nano-particle (GNP, 20 nm) for the monitoring cellular protein kinase activities. If cationic peptides, which are designed as specific substrates to target protein kinases, were added into the GNP dispersion, the color of the dispersion changed from red to blue due to the aggregation. On the other hand, if the peptide was phosphorylated with target protein kinase, addition of the phosphorylated peptide didn't change the color of the GNP dispersion. Thus, this system can be a rapid assay of protein kinase activity. The method was applicable to the assay of intracellular protein kinase by using cell lysate. In addition, we applied the system for high-throughput screening of protein kinase inhibitor using 1300 kinds of chemical library. The result indicated that the method was also useful for the drug discovery.

    Original languageEnglish
    Title of host publicationBiological Imaging and Sensing Using Nanoparticle Assemblies
    Pages39-44
    Number of pages6
    Publication statusPublished - Oct 15 2010
    Event2009 MRS Fall Meeting - Boston, MA, United States
    Duration: Nov 30 2009Dec 4 2009

    Publication series

    NameMaterials Research Society Symposium Proceedings
    Volume1241
    ISSN (Print)0272-9172

    Other

    Other2009 MRS Fall Meeting
    Country/TerritoryUnited States
    CityBoston, MA
    Period11/30/0912/4/09

    All Science Journal Classification (ASJC) codes

    • Materials Science(all)
    • Condensed Matter Physics
    • Mechanics of Materials
    • Mechanical Engineering

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