High-throughput injection by circulating plasma-bubbles laden flows

Keishi Ohtonari, Yuta Arakawa, Hiroki Ogata, Daisuke Tujimoto, Yoko Yamanishi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We have succeeded in injection of plasmid to adherent cells which are suspended in the plasma-bubbles laden circulation flow in a chamber. High-speed plasma-bubbles are generated by glass electrode and the air-liquid interface has a stiction force which draws the gene (plasmid) and stick to the air-liquid interface. The circulating flow increased the chances for cells to contact air-liquid interface of bubbles which is enclosing plasma or reactive gas. Finally, the high reactive interface enables gene transfer to cells efficiently. This proposed two dimensional microfluidic chip contributes to high-throughput gene transfer in bio-medical applications.

Original languageEnglish
Title of host publication2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages144-147
Number of pages4
ISBN (Electronic)9781509019472
DOIs
Publication statusPublished - Nov 28 2016
Externally publishedYes
Event11th IEEE Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016 - Sendai, Japan
Duration: Apr 17 2016Apr 20 2016

Other

Other11th IEEE Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016
CountryJapan
CitySendai
Period4/17/164/20/16

Fingerprint

Gene transfer
Throughput
Plasmas
Liquids
Plasmids
Air
Stiction
Medical applications
Bubbles (in fluids)
Microfluidics
Contacts (fluid mechanics)
Genes
Gases
Glass
Electrodes

All Science Journal Classification (ASJC) codes

  • Industrial and Manufacturing Engineering
  • Mechanical Engineering
  • Mechanics of Materials
  • Electronic, Optical and Magnetic Materials

Cite this

Ohtonari, K., Arakawa, Y., Ogata, H., Tujimoto, D., & Yamanishi, Y. (2016). High-throughput injection by circulating plasma-bubbles laden flows. In 2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016 (pp. 144-147). [7758218] Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/NEMS.2016.7758218

High-throughput injection by circulating plasma-bubbles laden flows. / Ohtonari, Keishi; Arakawa, Yuta; Ogata, Hiroki; Tujimoto, Daisuke; Yamanishi, Yoko.

2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016. Institute of Electrical and Electronics Engineers Inc., 2016. p. 144-147 7758218.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Ohtonari, K, Arakawa, Y, Ogata, H, Tujimoto, D & Yamanishi, Y 2016, High-throughput injection by circulating plasma-bubbles laden flows. in 2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016., 7758218, Institute of Electrical and Electronics Engineers Inc., pp. 144-147, 11th IEEE Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016, Sendai, Japan, 4/17/16. https://doi.org/10.1109/NEMS.2016.7758218
Ohtonari K, Arakawa Y, Ogata H, Tujimoto D, Yamanishi Y. High-throughput injection by circulating plasma-bubbles laden flows. In 2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016. Institute of Electrical and Electronics Engineers Inc. 2016. p. 144-147. 7758218 https://doi.org/10.1109/NEMS.2016.7758218
Ohtonari, Keishi ; Arakawa, Yuta ; Ogata, Hiroki ; Tujimoto, Daisuke ; Yamanishi, Yoko. / High-throughput injection by circulating plasma-bubbles laden flows. 2016 IEEE 11th Annual International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2016. Institute of Electrical and Electronics Engineers Inc., 2016. pp. 144-147
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