TY - GEN
T1 - High throughput mechanical characterization of oocyte using robot integrated microfluidic chip
AU - Sakuma, Shinya
AU - Turan, Bilal
AU - Arai, Fumihito
N1 - Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.
PY - 2013
Y1 - 2013
N2 - This paper presents a novel measurement system of cellular mechanical properties based on a robot integrated microfluidic chip. In order to achieve the high throughput measurement of cellular mechacal properties, we proposed the robot integrated microfluidic chip (robochip), taking advantages of both of micromechanical manipulator and Lab-on-a-Chip devices. The robochip contained a pair of a magnetically driven on-chip robotic probe and a force sensor. The characterization system based on the robochip performed by the visual feedback control, and the continuous cell measurement was demonstrated. The throughput of our system was 15 to 20 seconds per one oocyte. Moreover, the measurement of the viscoelastic properties were demonstrated as a quality evaluation of oocyte. Experimental results shows that the oocyte has the viscoelastic properties among the same culture condition, and it is important to analyze the mechanical properties of oocyte for the evaluation of the quality. From these results, we concluded that the high through put cellular mechanical characterization was achieved, and our robochip approach was a promising technique for a cellular characterization because the chip part was disposable.
AB - This paper presents a novel measurement system of cellular mechanical properties based on a robot integrated microfluidic chip. In order to achieve the high throughput measurement of cellular mechacal properties, we proposed the robot integrated microfluidic chip (robochip), taking advantages of both of micromechanical manipulator and Lab-on-a-Chip devices. The robochip contained a pair of a magnetically driven on-chip robotic probe and a force sensor. The characterization system based on the robochip performed by the visual feedback control, and the continuous cell measurement was demonstrated. The throughput of our system was 15 to 20 seconds per one oocyte. Moreover, the measurement of the viscoelastic properties were demonstrated as a quality evaluation of oocyte. Experimental results shows that the oocyte has the viscoelastic properties among the same culture condition, and it is important to analyze the mechanical properties of oocyte for the evaluation of the quality. From these results, we concluded that the high through put cellular mechanical characterization was achieved, and our robochip approach was a promising technique for a cellular characterization because the chip part was disposable.
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U2 - 10.1109/IROS.2013.6696630
DO - 10.1109/IROS.2013.6696630
M3 - Conference contribution
AN - SCOPUS:84893724889
SN - 9781467363587
T3 - IEEE International Conference on Intelligent Robots and Systems
SP - 2047
EP - 2052
BT - IROS 2013
T2 - 2013 26th IEEE/RSJ International Conference on Intelligent Robots and Systems: New Horizon, IROS 2013
Y2 - 3 November 2013 through 8 November 2013
ER -