High-throughput methylation mapping by detecting fluorescently stained methylation sites at a single molecule level

Atsunori Hattori, Takao Yasui, Noritada Kaji, Yoshinobu Baba

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We detected methylation sites on elongated lambda DNA molecules as high fluorescent distinct peaks via double collar imaging. We used methyl-CpG-binding domain 2 protein (MBD2p) combined with the quantum dots (QDs) for methylation imaging, and YOYO-1 for DNA imaging.

Original languageEnglish
Title of host publicationMicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages861-863
Number of pages3
ISBN (Electronic)9780979806483
Publication statusPublished - Jan 1 2015
Externally publishedYes
Event19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, Korea, Republic of
Duration: Oct 25 2015Oct 29 2015

Publication series

NameMicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Other

Other19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015
CountryKorea, Republic of
CityGyeongju
Period10/25/1510/29/15

Fingerprint

Methylation
Throughput
Imaging techniques
Molecules
DNA
Semiconductor quantum dots
Proteins

All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

Cite this

Hattori, A., Yasui, T., Kaji, N., & Baba, Y. (2015). High-throughput methylation mapping by detecting fluorescently stained methylation sites at a single molecule level. In MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 861-863). (MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences). Chemical and Biological Microsystems Society.

High-throughput methylation mapping by detecting fluorescently stained methylation sites at a single molecule level. / Hattori, Atsunori; Yasui, Takao; Kaji, Noritada; Baba, Yoshinobu.

MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2015. p. 861-863 (MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Hattori, A, Yasui, T, Kaji, N & Baba, Y 2015, High-throughput methylation mapping by detecting fluorescently stained methylation sites at a single molecule level. in MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Chemical and Biological Microsystems Society, pp. 861-863, 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015, Gyeongju, Korea, Republic of, 10/25/15.
Hattori A, Yasui T, Kaji N, Baba Y. High-throughput methylation mapping by detecting fluorescently stained methylation sites at a single molecule level. In MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society. 2015. p. 861-863. (MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences).
Hattori, Atsunori ; Yasui, Takao ; Kaji, Noritada ; Baba, Yoshinobu. / High-throughput methylation mapping by detecting fluorescently stained methylation sites at a single molecule level. MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2015. pp. 861-863 (MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences).
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