TY - JOUR
T1 - High-throughput phospholipid profiling system based on supercritical fluid extraction-supercritical fluid chromatography/mass spectrometry for dried plasma spot analysis
AU - Uchikata, Takato
AU - Matsubara, Atsuki
AU - Fukusaki, Eiichiro
AU - Bamba, Takeshi
N1 - Funding Information:
This research was partially supported by KAKENHI [Grant-in-Aid for Young Scientists (A) 23686120 and (B) 10432444], Suntory Institute for Bioorganic Research .
PY - 2012/8/10
Y1 - 2012/8/10
N2 - The dried blood spots (DBS) and dried plasma spots (DPS) analysis is available as a diagnostic tool for genetic diseases and is useful for the screening of biomarkers. In this study, a high-throughput analytical system based on supercritical fluid extraction-supercritical fluid chromatography with tandem mass spectrometry (SFE-SFC/MS/MS) was constructed for phospholipids profiling of DPS. This system is able to simultaneously perform extraction and separation, allowing phospholipids that have common polar head groups to be analyzed. Phospholipids in only 3 μl of plasma can be extracted in 5. min and analyzed within 15. min using this system. A total of 134 phospholipids, including phosphatidylcholine, lysophosphatidylcholine, sphingomyelin, phosphatidylethanolamine and lysophosphatidylethanolamine, were annotated, and 74 phospholipids were analyzed with good repeatability. The SFE-SFC/MS/MS, which is able to perform high-throughput lipid profiling analysis for clinical diagnosis and drug discovery, may also be suitable for the screening of biomarkers.
AB - The dried blood spots (DBS) and dried plasma spots (DPS) analysis is available as a diagnostic tool for genetic diseases and is useful for the screening of biomarkers. In this study, a high-throughput analytical system based on supercritical fluid extraction-supercritical fluid chromatography with tandem mass spectrometry (SFE-SFC/MS/MS) was constructed for phospholipids profiling of DPS. This system is able to simultaneously perform extraction and separation, allowing phospholipids that have common polar head groups to be analyzed. Phospholipids in only 3 μl of plasma can be extracted in 5. min and analyzed within 15. min using this system. A total of 134 phospholipids, including phosphatidylcholine, lysophosphatidylcholine, sphingomyelin, phosphatidylethanolamine and lysophosphatidylethanolamine, were annotated, and 74 phospholipids were analyzed with good repeatability. The SFE-SFC/MS/MS, which is able to perform high-throughput lipid profiling analysis for clinical diagnosis and drug discovery, may also be suitable for the screening of biomarkers.
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U2 - 10.1016/j.chroma.2012.06.031
DO - 10.1016/j.chroma.2012.06.031
M3 - Article
C2 - 22749580
AN - SCOPUS:84863871832
VL - 1250
SP - 69
EP - 75
JO - Journal of Chromatography A
JF - Journal of Chromatography A
SN - 0021-9673
ER -