High throughput purification device for gene delivery multifunctional envelope-type nanodevice

Daisuke Shigenaka, Masami Ukawa, Noritada Kaji, Yukihiro Okamoto, Manabu Tokeshi, Hidetaka Akita, Hideyoshi Harashima, Yoshinobu Baba

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Multifunctional envelope-type nanodevices (MEND) developed by Harashima et al. is one of the novel non-viral DNA vectors expected as a safe gene delivery system. Our group has already attained fast and easy preparation of MEND by using the microfluidic device. However, purification of MEND should be performed in a high-throughput manner because prepared MEND contains impurities such as plasmid-cationic polymer complex. In this paper, we report a novel purification method for MEND by free flow electrophoresis based on microfluidic device, which purification principle is based on the zeta potential difference between MEND and impurities.

Original languageEnglish
Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PublisherChemical and Biological Microsystems Society
Pages1546-1548
Number of pages3
ISBN (Print)9780979806452
Publication statusPublished - Jan 1 2012
Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
Duration: Oct 28 2012Nov 1 2012

Publication series

NameProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

Other

Other16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
CountryJapan
CityOkinawa
Period10/28/1211/1/12

Fingerprint

Purification
Genes
Throughput
Microfluidics
Impurities
Zeta potential
Electrophoresis
Polymers
Plasmids
DNA

All Science Journal Classification (ASJC) codes

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Cite this

Shigenaka, D., Ukawa, M., Kaji, N., Okamoto, Y., Tokeshi, M., Akita, H., ... Baba, Y. (2012). High throughput purification device for gene delivery multifunctional envelope-type nanodevice. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 (pp. 1546-1548). (Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012). Chemical and Biological Microsystems Society.

High throughput purification device for gene delivery multifunctional envelope-type nanodevice. / Shigenaka, Daisuke; Ukawa, Masami; Kaji, Noritada; Okamoto, Yukihiro; Tokeshi, Manabu; Akita, Hidetaka; Harashima, Hideyoshi; Baba, Yoshinobu.

Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. p. 1546-1548 (Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Shigenaka, D, Ukawa, M, Kaji, N, Okamoto, Y, Tokeshi, M, Akita, H, Harashima, H & Baba, Y 2012, High throughput purification device for gene delivery multifunctional envelope-type nanodevice. in Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, Chemical and Biological Microsystems Society, pp. 1546-1548, 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, Okinawa, Japan, 10/28/12.
Shigenaka D, Ukawa M, Kaji N, Okamoto Y, Tokeshi M, Akita H et al. High throughput purification device for gene delivery multifunctional envelope-type nanodevice. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society. 2012. p. 1546-1548. (Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012).
Shigenaka, Daisuke ; Ukawa, Masami ; Kaji, Noritada ; Okamoto, Yukihiro ; Tokeshi, Manabu ; Akita, Hidetaka ; Harashima, Hideyoshi ; Baba, Yoshinobu. / High throughput purification device for gene delivery multifunctional envelope-type nanodevice. Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. pp. 1546-1548 (Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012).
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