High throughput purification devices for in vivo applications of gene-delivery multifunctional envelope-type nanodevices

Noritada Kaji, Daisuke Shigenaka, Masami Ukawa, Manabu Tokeshi, Hidetaka Akita, Hideyoshi Harashima, Yoshinobu Baba

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We developed microfluidic continuous purification devices based on free-flow electrophoresis for gene-delivery multifunctional envelope-type nanodevices (MEND) which consists of DNA core and phospholipid bilayer envelope. Various impurities, such as DNA, DNA-peptide complex, and liposomes, produced during the fabrication process were removed in an efficient manner and over 75% collection of the input plasmid DNA which corresponds to purified MEND was achieved.

Original languageEnglish
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages1170-1172
Number of pages3
ISBN (Print)9781632666246
Publication statusPublished - Jan 1 2013
Externally publishedYes
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
Duration: Oct 27 2013Oct 31 2013

Publication series

Name17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Volume2

Other

Other17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
CountryGermany
CityFreiburg
Period10/27/1310/31/13

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All Science Journal Classification (ASJC) codes

  • Bioengineering

Cite this

Kaji, N., Shigenaka, D., Ukawa, M., Tokeshi, M., Akita, H., Harashima, H., & Baba, Y. (2013). High throughput purification devices for in vivo applications of gene-delivery multifunctional envelope-type nanodevices. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 (pp. 1170-1172). (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Vol. 2). Chemical and Biological Microsystems Society.