TY - GEN
T1 - High throughput purification devices for in vivo applications of gene-delivery multifunctional envelope-type nanodevices
AU - Kaji, Noritada
AU - Shigenaka, Daisuke
AU - Ukawa, Masami
AU - Tokeshi, Manabu
AU - Akita, Hidetaka
AU - Harashima, Hideyoshi
AU - Baba, Yoshinobu
PY - 2013/1/1
Y1 - 2013/1/1
N2 - We developed microfluidic continuous purification devices based on free-flow electrophoresis for gene-delivery multifunctional envelope-type nanodevices (MEND) which consists of DNA core and phospholipid bilayer envelope. Various impurities, such as DNA, DNA-peptide complex, and liposomes, produced during the fabrication process were removed in an efficient manner and over 75% collection of the input plasmid DNA which corresponds to purified MEND was achieved.
AB - We developed microfluidic continuous purification devices based on free-flow electrophoresis for gene-delivery multifunctional envelope-type nanodevices (MEND) which consists of DNA core and phospholipid bilayer envelope. Various impurities, such as DNA, DNA-peptide complex, and liposomes, produced during the fabrication process were removed in an efficient manner and over 75% collection of the input plasmid DNA which corresponds to purified MEND was achieved.
UR - http://www.scopus.com/inward/record.url?scp=84907362892&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84907362892&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84907362892
SN - 9781632666246
T3 - 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
SP - 1170
EP - 1172
BT - 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PB - Chemical and Biological Microsystems Society
T2 - 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Y2 - 27 October 2013 through 31 October 2013
ER -