High-throughput simultaneous analysis of pesticides by supercritical fluid chromatography/tandem mass spectrometry

Megumi Ishibashi, Takashi Ando, Miho Sakai, Atsuki Matsubara, Takato Uchikata, Eiichiro Fukusaki, Takeshi Bamba

Research output: Contribution to journalArticle

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Abstract

Combination techniques such as gas chromatography/mass spectrometry (GC/MS) and liquid chromatography/mass spectrometry (LC/MS) are commonly used for pesticide residue analysis, but there is no reported method for the simultaneous analysis of multiple pesticides in a sample using a single instrument. Supercritical fluid chromatography (SFC) offers high resolution at high flow rates and various separation modes and hence may aid the rapid simultaneous analysis of pesticide. We developed an SFC/MS/MS method and analyzed 17 pesticides with a wide range of polarities (logPow=-4.6 to 7.05) and molecular weights (112.1-888.6) within 11min using a polar-embedded reversed-phase column. To the best of our knowledge, there is no previous report on the SFC analysis of a wide variety of compounds, including highly hydrophilic ones. By SFC, diquat dibromide (logPow=-4.6), together with cypermethrin (logPow=6.6) and tralomethrin (logPow=5.05), could be detected in the presence of various other pesticides using a single mobile phase. SFC/MS allows for the rapid and simultaneous analysis of low concentrations (ng/L levels) of pesticides that typically need to be analyzed by GC/MS and LC/MS separately.

Original languageEnglish
Pages (from-to)143-148
Number of pages6
JournalJournal of Chromatography A
Volume1266
DOIs
Publication statusPublished - Nov 30 2012
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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