High-throughput study of protein-surface interactions using a surface plasmon resonance imaging apparatus

Yusuke Arima; Rika Ishii; Isao Hirata; Hiroo Iwata

doi:10.1021/bk-2012-1120.ch032

High-throughput study of protein-surface interactions using a surface plasmon resonance imaging apparatus

Yusuke Arima, Rika Ishii, Isao Hirata, Hiroo Iwata

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

1 Citation (Scopus)

Abstract

Protein adsorption at the liquid-solid interface is critically important for the development of biomedical materials. Surface plasmon resonance (SPR) has been widely used in real-time monitoring of protein adsorption behavior on surfaces. However, the number of combinations of protein-surface interactions that can be measured in a single experiment is limited by a conventional SPR apparatus. We assembled an SPR imaging apparatus for high-throughput studies of many sets of protein-surface interactions. In addition, SPR sensor surfaces with arrayed spots carrying different surface properties were prepared by taking advantage of self-assembled monolayers of alkanethiols. The combination of the SPR imaging apparatus and the arrayed sensor surfaces allows for multiple and real-time monitoring of protein-surface interactions.

Original language	English
Title of host publication	Proteins at Interfaces III State of the Art
Publisher	American Chemical Society
Pages	695-708
Number of pages	14
ISBN (Print)	9780841227965
DOIs	https://doi.org/10.1021/bk-2012-1120.ch032
Publication status	Published - Dec 12 2012
Externally published	Yes

Publication series

Name	ACS Symposium Series
Volume	1120
ISSN (Print)	0097-6156
ISSN (Electronic)	1947-5918

All Science Journal Classification (ASJC) codes

Chemistry(all)
Chemical Engineering(all)

Access to Document

10.1021/bk-2012-1120.ch032

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High-throughput study of protein-surface interactions using a surface plasmon resonance imaging apparatus. / Arima, Yusuke; Ishii, Rika; Hirata, Isao; Iwata, Hiroo.

Proteins at Interfaces III State of the Art. American Chemical Society, 2012. p. 695-708 (ACS Symposium Series; Vol. 1120).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

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