High-throughput study of protein-surface interactions using a surface plasmon resonance imaging apparatus

Yusuke Arima, Rika Ishii, Isao Hirata, Hiroo Iwata

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Protein adsorption at the liquid-solid interface is critically important for the development of biomedical materials. Surface plasmon resonance (SPR) has been widely used in real-time monitoring of protein adsorption behavior on surfaces. However, the number of combinations of protein-surface interactions that can be measured in a single experiment is limited by a conventional SPR apparatus. We assembled an SPR imaging apparatus for high-throughput studies of many sets of protein-surface interactions. In addition, SPR sensor surfaces with arrayed spots carrying different surface properties were prepared by taking advantage of self-assembled monolayers of alkanethiols. The combination of the SPR imaging apparatus and the arrayed sensor surfaces allows for multiple and real-time monitoring of protein-surface interactions.

Original languageEnglish
Title of host publicationProteins at Interfaces III State of the Art
PublisherAmerican Chemical Society
Pages695-708
Number of pages14
ISBN (Print)9780841227965
DOIs
Publication statusPublished - Dec 12 2012

Publication series

NameACS Symposium Series
Volume1120
ISSN (Print)0097-6156
ISSN (Electronic)1947-5918

Fingerprint

Surface plasmon resonance
Membrane Proteins
Throughput
Proteins
Imaging techniques
Adsorption
Monitoring
Sensors
Self assembled monolayers
Surface properties
Liquids
Experiments

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

Arima, Y., Ishii, R., Hirata, I., & Iwata, H. (2012). High-throughput study of protein-surface interactions using a surface plasmon resonance imaging apparatus. In Proteins at Interfaces III State of the Art (pp. 695-708). (ACS Symposium Series; Vol. 1120). American Chemical Society. https://doi.org/10.1021/bk-2012-1120.ch032

High-throughput study of protein-surface interactions using a surface plasmon resonance imaging apparatus. / Arima, Yusuke; Ishii, Rika; Hirata, Isao; Iwata, Hiroo.

Proteins at Interfaces III State of the Art. American Chemical Society, 2012. p. 695-708 (ACS Symposium Series; Vol. 1120).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Arima, Y, Ishii, R, Hirata, I & Iwata, H 2012, High-throughput study of protein-surface interactions using a surface plasmon resonance imaging apparatus. in Proteins at Interfaces III State of the Art. ACS Symposium Series, vol. 1120, American Chemical Society, pp. 695-708. https://doi.org/10.1021/bk-2012-1120.ch032
Arima Y, Ishii R, Hirata I, Iwata H. High-throughput study of protein-surface interactions using a surface plasmon resonance imaging apparatus. In Proteins at Interfaces III State of the Art. American Chemical Society. 2012. p. 695-708. (ACS Symposium Series). https://doi.org/10.1021/bk-2012-1120.ch032
Arima, Yusuke ; Ishii, Rika ; Hirata, Isao ; Iwata, Hiroo. / High-throughput study of protein-surface interactions using a surface plasmon resonance imaging apparatus. Proteins at Interfaces III State of the Art. American Chemical Society, 2012. pp. 695-708 (ACS Symposium Series).
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