High-throughput study of protein-surface interactions using a surface plasmon resonance imaging apparatus

Yusuke Arima, Rika Ishii, Isao Hirata, Hiroo Iwata

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Protein adsorption at the liquid-solid interface is critically important for the development of biomedical materials. Surface plasmon resonance (SPR) has been widely used in real-time monitoring of protein adsorption behavior on surfaces. However, the number of combinations of protein-surface interactions that can be measured in a single experiment is limited by a conventional SPR apparatus. We assembled an SPR imaging apparatus for high-throughput studies of many sets of protein-surface interactions. In addition, SPR sensor surfaces with arrayed spots carrying different surface properties were prepared by taking advantage of self-assembled monolayers of alkanethiols. The combination of the SPR imaging apparatus and the arrayed sensor surfaces allows for multiple and real-time monitoring of protein-surface interactions.

Original languageEnglish
Title of host publicationProteins at Interfaces III State of the Art
PublisherAmerican Chemical Society
Pages695-708
Number of pages14
ISBN (Print)9780841227965
DOIs
Publication statusPublished - Dec 12 2012
Externally publishedYes

Publication series

NameACS Symposium Series
Volume1120
ISSN (Print)0097-6156
ISSN (Electronic)1947-5918

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)

Fingerprint Dive into the research topics of 'High-throughput study of protein-surface interactions using a surface plasmon resonance imaging apparatus'. Together they form a unique fingerprint.

Cite this