High-throughput technique for comprehensive analysis of Japanese green tea quality assessment using ultra-performance liquid chromatography with time-of-flight mass spectrometry (UPLC/TOF MS)

Wipawee Pongsuwan, Takeshi Bamba, Kazuo Harada, Tsutomu Yonetani, Akio Kobayashi, Eiichiro Fukusaki

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

Applications of metabolomics techniques along with chemometrics provide an understanding in the relationship between metabolome of green tea and its quality. A coupled of ultra-performance liquid chromatography with time-of-flight mass spectrometry (UPLC/TOF MS) allowed a high-throughput and comprehensive analysis with minimal sample preparation. Using this technique, a wide range of metabolites were investigated. Data analysis was rapid, considering that the fingerprinting technique was performed. A set of green tea samples from 2006 tea contest of the Kansai area was analyzed to prove usefulness of the developed technique. Green tea with different qualities were discriminated through principal component analysis (PCA). Consequently, projection to latent structure by means of partial least-squares (PLS) was performed to create a constructive quality-predictive model by means of metabolic fingerprinting. Beside epigallocatechin, other predominant catechins, including epigallocatechin gallate and epicatechin gallate, detected in green tea were found to be significant biomarkers to the high quality of Japanese green tea (Sencha).

Original languageEnglish
Pages (from-to)10705-10708
Number of pages4
JournalJournal of Agricultural and Food Chemistry
Volume56
Issue number22
DOIs
Publication statusPublished - Nov 26 2008
Externally publishedYes

Fingerprint

ultra-performance liquid chromatography
Liquid chromatography
green tea
Tea
Liquid Chromatography
Mass spectrometry
Mass Spectrometry
Throughput
mass spectrometry
epigallocatechin
metabolome
methodology
chemometrics
epicatechin
metabolomics
flavanols
Metabolomics
Metabolome
Catechin
tea

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)

Cite this

High-throughput technique for comprehensive analysis of Japanese green tea quality assessment using ultra-performance liquid chromatography with time-of-flight mass spectrometry (UPLC/TOF MS). / Pongsuwan, Wipawee; Bamba, Takeshi; Harada, Kazuo; Yonetani, Tsutomu; Kobayashi, Akio; Fukusaki, Eiichiro.

In: Journal of Agricultural and Food Chemistry, Vol. 56, No. 22, 26.11.2008, p. 10705-10708.

Research output: Contribution to journalArticle

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AU - Harada, Kazuo

AU - Yonetani, Tsutomu

AU - Kobayashi, Akio

AU - Fukusaki, Eiichiro

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