Highly efficient continuous acetone–butanol–ethanol production from mixed sugars without carbon catabolite repression

Takahiro Kihara, Takuya Noguchi, Yukihiro Tashiro, Kenji Sakai, Kenji Sonomoto

Research output: Contribution to journalArticle

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Abstract

Factors including the composition and concentration of mixed sugars, dilution rate, and cell density were examined to establish a highly efficient continuous acetone–butanol–ethanol (ABE)fermentation process using Clostridium saccharoperbutylacetonicum N1-4. During conventional continuous fermentation at a dilution rate of 0.15 h−1, the glucose/xylose mixture exhibited carbon catabolite repression (CCR)with an intensity (I CCR)of 4.18 and ABE productivity of 0.574 g L−1 h−1, while CCR was alleviated using a cellobiose/xylose mixture with 1.60 and 1.09 g L−1 h−1, respectively. Continuous fermentation with high cell density by cell recycling improved ABE productivity to 5.32 g L−1 h−1 at a dilution rate of 0.686 h−1 owing to high xylose consumption. Further optimization of mixed sugar concentration in the feeding medium induced the best performance of continuous ABE production from mixed sugar without CCR, relative to previous studies: ABE productivity, 6.09 g L−1 h−1; ABE yield, 0.214 g g−1; I CCR, 1.48.

Original languageEnglish
Article number100185
JournalBioresource Technology Reports
Volume7
DOIs
Publication statusPublished - Sep 2019

Fingerprint

Sugars
sugar
Carbon
Xylose
Fermentation
carbon
Dilution
fermentation
dilution
Productivity
productivity
Cellobiose
Clostridium
Glucose
Recycling
glucose
recycling
repression
Chemical analysis
rate

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Renewable Energy, Sustainability and the Environment
  • Environmental Engineering
  • Waste Management and Disposal

Cite this

Highly efficient continuous acetone–butanol–ethanol production from mixed sugars without carbon catabolite repression. / Kihara, Takahiro; Noguchi, Takuya; Tashiro, Yukihiro; Sakai, Kenji; Sonomoto, Kenji.

In: Bioresource Technology Reports, Vol. 7, 100185, 09.2019.

Research output: Contribution to journalArticle

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