Abstract
Factors including the composition and concentration of mixed sugars, dilution rate, and cell density were examined to establish a highly efficient continuous acetone–butanol–ethanol (ABE)fermentation process using Clostridium saccharoperbutylacetonicum N1-4. During conventional continuous fermentation at a dilution rate of 0.15 h−1, the glucose/xylose mixture exhibited carbon catabolite repression (CCR)with an intensity (I CCR)of 4.18 and ABE productivity of 0.574 g L−1 h−1, while CCR was alleviated using a cellobiose/xylose mixture with 1.60 and 1.09 g L−1 h−1, respectively. Continuous fermentation with high cell density by cell recycling improved ABE productivity to 5.32 g L−1 h−1 at a dilution rate of 0.686 h−1 owing to high xylose consumption. Further optimization of mixed sugar concentration in the feeding medium induced the best performance of continuous ABE production from mixed sugar without CCR, relative to previous studies: ABE productivity, 6.09 g L−1 h−1; ABE yield, 0.214 g g−1; I CCR, 1.48.
| Original language | English |
|---|---|
| Article number | 100185 |
| Journal | Bioresource Technology Reports |
| Volume | 7 |
| DOIs | |
| Publication status | Published - Sep 2019 |
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All Science Journal Classification (ASJC) codes
- Bioengineering
- Renewable Energy, Sustainability and the Environment
- Environmental Engineering
- Waste Management and Disposal
Cite this
Highly efficient continuous acetone–butanol–ethanol production from mixed sugars without carbon catabolite repression. / Kihara, Takahiro; Noguchi, Takuya; Tashiro, Yukihiro; Sakai, Kenji; Sonomoto, Kenji.
In: Bioresource Technology Reports, Vol. 7, 100185, 09.2019.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Highly efficient continuous acetone–butanol–ethanol production from mixed sugars without carbon catabolite repression
AU - Kihara, Takahiro
AU - Noguchi, Takuya
AU - Tashiro, Yukihiro
AU - Sakai, Kenji
AU - Sonomoto, Kenji
PY - 2019/9
Y1 - 2019/9
N2 - Factors including the composition and concentration of mixed sugars, dilution rate, and cell density were examined to establish a highly efficient continuous acetone–butanol–ethanol (ABE)fermentation process using Clostridium saccharoperbutylacetonicum N1-4. During conventional continuous fermentation at a dilution rate of 0.15 h−1, the glucose/xylose mixture exhibited carbon catabolite repression (CCR)with an intensity (I CCR)of 4.18 and ABE productivity of 0.574 g L−1 h−1, while CCR was alleviated using a cellobiose/xylose mixture with 1.60 and 1.09 g L−1 h−1, respectively. Continuous fermentation with high cell density by cell recycling improved ABE productivity to 5.32 g L−1 h−1 at a dilution rate of 0.686 h−1 owing to high xylose consumption. Further optimization of mixed sugar concentration in the feeding medium induced the best performance of continuous ABE production from mixed sugar without CCR, relative to previous studies: ABE productivity, 6.09 g L−1 h−1; ABE yield, 0.214 g g−1; I CCR, 1.48.
AB - Factors including the composition and concentration of mixed sugars, dilution rate, and cell density were examined to establish a highly efficient continuous acetone–butanol–ethanol (ABE)fermentation process using Clostridium saccharoperbutylacetonicum N1-4. During conventional continuous fermentation at a dilution rate of 0.15 h−1, the glucose/xylose mixture exhibited carbon catabolite repression (CCR)with an intensity (I CCR)of 4.18 and ABE productivity of 0.574 g L−1 h−1, while CCR was alleviated using a cellobiose/xylose mixture with 1.60 and 1.09 g L−1 h−1, respectively. Continuous fermentation with high cell density by cell recycling improved ABE productivity to 5.32 g L−1 h−1 at a dilution rate of 0.686 h−1 owing to high xylose consumption. Further optimization of mixed sugar concentration in the feeding medium induced the best performance of continuous ABE production from mixed sugar without CCR, relative to previous studies: ABE productivity, 6.09 g L−1 h−1; ABE yield, 0.214 g g−1; I CCR, 1.48.
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UR - http://www.scopus.com/inward/citedby.url?scp=85075409735&partnerID=8YFLogxK
U2 - 10.1016/j.biteb.2019.03.017
DO - 10.1016/j.biteb.2019.03.017
M3 - Article
AN - SCOPUS:85075409735
VL - 7
JO - Bioresource Technology Reports
JF - Bioresource Technology Reports
SN - 2589-014X
M1 - 100185
ER -