TY - JOUR
T1 - Highly sensitive detection of 2,4,6-trinitrotoluene(TNT) using poly(vinylamine-co-N-vinylformamide)based surface plasmon resonance (SPR) immunosensor
AU - Yatabe, Rui
AU - Onodera, Takeshi
AU - Toko, Kiyoshi
PY - 2013
Y1 - 2013
N2 - In this paper, we describe the fabrication of a novel poly-(vinylamine-co- Nvinylformamide) (poly-(VAm-co-NVF))-based surface plasmon resonance (SPR) sensor chip supported by a self-assembled monolayer (SAM) of alkanethiol and its use in the highly sensitive detection of 2,4,6-trinitrotoluene (TNT), which is a typical explosive. 2,4-dinitrophenyl-glycine (DNP-GIy) was immobilized to amino groups on the side chain of poly-vinylamine (poly-VAm) on the sensor surface. The fabricated surface showed a high response for flow of anti-TNT antibodies. However, it was found that the response included the nonspecific adsorption of anti-TNT antibodies, because unreacted amino groups of the poly-VAm were positively charged and it caused nonspecific adsorption due to electrostatic interaction. Then, the number of amino groups was reduced by making low-hydrolyzed poly-N-vinylformamideipoly-NVF), i.e., poly(VAm-co-NVF) -poly-NVF is a precursor of poly-VAm, and nonspecific adsorption was controlled. The limit of detection (LOD) of TNT was 28 ppt using the sensor chip fabricated with 23% hydrolyzed poly-(VAm-co-NVF) by inhibition assay.
AB - In this paper, we describe the fabrication of a novel poly-(vinylamine-co- Nvinylformamide) (poly-(VAm-co-NVF))-based surface plasmon resonance (SPR) sensor chip supported by a self-assembled monolayer (SAM) of alkanethiol and its use in the highly sensitive detection of 2,4,6-trinitrotoluene (TNT), which is a typical explosive. 2,4-dinitrophenyl-glycine (DNP-GIy) was immobilized to amino groups on the side chain of poly-vinylamine (poly-VAm) on the sensor surface. The fabricated surface showed a high response for flow of anti-TNT antibodies. However, it was found that the response included the nonspecific adsorption of anti-TNT antibodies, because unreacted amino groups of the poly-VAm were positively charged and it caused nonspecific adsorption due to electrostatic interaction. Then, the number of amino groups was reduced by making low-hydrolyzed poly-N-vinylformamideipoly-NVF), i.e., poly(VAm-co-NVF) -poly-NVF is a precursor of poly-VAm, and nonspecific adsorption was controlled. The limit of detection (LOD) of TNT was 28 ppt using the sensor chip fabricated with 23% hydrolyzed poly-(VAm-co-NVF) by inhibition assay.
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M3 - Article
AN - SCOPUS:84874549365
VL - 25
SP - 45
EP - 56
JO - Sensors and Materials
JF - Sensors and Materials
SN - 0914-4935
IS - 1
ER -