Highly sensitive detection of TNT using a poly(amidoamine) dendron-based SPR immunosensor

Yutaka Mizuta, Takeshi Onodera, Praveen Singh, Kiyoshi Matsumoto, Norio Miura, Kiyoshi Toko

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

In this paper, we describe the fabrication of novel poly(amidoamine) (PAMAM) dendron-based Surface Plasmon Resonance (SPR) sensor chip surfaces supported by a self-assembled monolayer (SAM) of alkanethiol and their use in the highly sensitive detection of 2,4,6-trinitrotoluene (TNT), which is one of main components of explosives. The fabricated surfaces showed excellent binding capacity for TNT antibodies; this is probably due to the fact that the highly branched functional groups and three-dimensional structure of dendron molecules offer densely arranged binding sites and improve binding efficiency. A limit of detection (LOD) of ca. 15 ppt (pg ml-1) was achieved by inhibition assay.

Original languageEnglish
Pages (from-to)193-200
Number of pages8
JournalSensors and Materials
Volume22
Issue number4
Publication statusPublished - Jul 15 2010

Fingerprint

Trinitrotoluene
Immunosensors
trinitrotoluene
Surface plasmon resonance
surface plasmon resonance
Self assembled monolayers
Binding sites
antibodies
Antibodies
Functional groups
Assays
Binding Sites
chips
Fabrication
fabrication
Molecules
sensors
Sensors
molecules
Poly(amidoamine)

All Science Journal Classification (ASJC) codes

  • Instrumentation
  • Materials Science(all)

Cite this

Mizuta, Y., Onodera, T., Singh, P., Matsumoto, K., Miura, N., & Toko, K. (2010). Highly sensitive detection of TNT using a poly(amidoamine) dendron-based SPR immunosensor. Sensors and Materials, 22(4), 193-200.

Highly sensitive detection of TNT using a poly(amidoamine) dendron-based SPR immunosensor. / Mizuta, Yutaka; Onodera, Takeshi; Singh, Praveen; Matsumoto, Kiyoshi; Miura, Norio; Toko, Kiyoshi.

In: Sensors and Materials, Vol. 22, No. 4, 15.07.2010, p. 193-200.

Research output: Contribution to journalArticle

Mizuta, Y, Onodera, T, Singh, P, Matsumoto, K, Miura, N & Toko, K 2010, 'Highly sensitive detection of TNT using a poly(amidoamine) dendron-based SPR immunosensor', Sensors and Materials, vol. 22, no. 4, pp. 193-200.
Mizuta, Yutaka ; Onodera, Takeshi ; Singh, Praveen ; Matsumoto, Kiyoshi ; Miura, Norio ; Toko, Kiyoshi. / Highly sensitive detection of TNT using a poly(amidoamine) dendron-based SPR immunosensor. In: Sensors and Materials. 2010 ; Vol. 22, No. 4. pp. 193-200.
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