Highly sensitive detection of TNT using a poly(amidoamine) dendron-based SPR immunosensor

Yutaka Mizuta, Takeshi Onodera, Praveen Singh, Kiyoshi Matsumoto, Norio Miura, Kiyoshi Toko

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

In this paper, we describe the fabrication of novel poly(amidoamine) (PAMAM) dendron-based Surface Plasmon Resonance (SPR) sensor chip surfaces supported by a self-assembled monolayer (SAM) of alkanethiol and their use in the highly sensitive detection of 2,4,6-trinitrotoluene (TNT), which is one of main components of explosives. The fabricated surfaces showed excellent binding capacity for TNT antibodies; this is probably due to the fact that the highly branched functional groups and three-dimensional structure of dendron molecules offer densely arranged binding sites and improve binding efficiency. A limit of detection (LOD) of ca. 15 ppt (pg ml-1) was achieved by inhibition assay.

Original languageEnglish
Pages (from-to)193-200
Number of pages8
JournalSensors and Materials
Volume22
Issue number4
Publication statusPublished - 2010

All Science Journal Classification (ASJC) codes

  • Instrumentation
  • Materials Science(all)

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