Highly Sensitive Simultaneous Determination of Prostaglandins by Using HPLC/Laser Induced Fluorescence (LIF).

Shinya Sakae, Akira Harata, Takehiko Kitamori, Tsuguo Sawada, Akira Okubo, Shozo Toda, Takao Shimizu, Hideo Watanabe

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We have developed a laser induced fluorometric (LIF) detection method combined with HPLC and an analytical procedure for prostaglandins (PGs) which allows analysis of practical samples without interference from admixtures and residual reagents. We were able to derivatize PGs with 9-anthryldiazomethtane (ADAM) at 4°C in the dark after 12 hours. This method was applied to analysis of human urine (20 μL). Clear separation and detection of PGs at fmole levels could be achieved. The lower detection limit was 1.0 fmole for PGF1α.

Original languageEnglish
Pages (from-to)957-958
Number of pages2
Journalanalytical sciences
Volume7
DOIs
Publication statusPublished - Jan 1991
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

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