Highly Sensitive Simultaneous Determination of Prostaglandins by Using HPLC/Laser Induced Fluorescence (LIF).

Shinya Sakae; Akira Harata; Takehiko Kitamori; Tsuguo Sawada; Akira Okubo; Shozo Toda; Takao Shimizu; Hideo Watanabe

doi:10.2116/analsci.7.Supple_957

Highly Sensitive Simultaneous Determination of Prostaglandins by Using HPLC/Laser Induced Fluorescence (LIF).

Shinya Sakae, Akira Harata, Takehiko Kitamori, Tsuguo Sawada, Akira Okubo, Shozo Toda, Takao Shimizu, Hideo Watanabe

Research output: Contribution to journal › Article › peer-review

Abstract

We have developed a laser induced fluorometric (LIF) detection method combined with HPLC and an analytical procedure for prostaglandins (PGs) which allows analysis of practical samples without interference from admixtures and residual reagents. We were able to derivatize PGs with 9-anthryldiazomethtane (ADAM) at 4°C in the dark after 12 hours. This method was applied to analysis of human urine (20 μL). Clear separation and detection of PGs at fmole levels could be achieved. The lower detection limit was 1.0 fmole for PGF1α.

Original language	English
Pages (from-to)	957-958
Number of pages	2
Journal	analytical sciences
Volume	7
DOIs	https://doi.org/10.2116/analsci.7.Supple_957
Publication status	Published - Jan 1991
Externally published	Yes

All Science Journal Classification (ASJC) codes

Analytical Chemistry

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title = "Highly Sensitive Simultaneous Determination of Prostaglandins by Using HPLC/Laser Induced Fluorescence (LIF).",

abstract = "We have developed a laser induced fluorometric (LIF) detection method combined with HPLC and an analytical procedure for prostaglandins (PGs) which allows analysis of practical samples without interference from admixtures and residual reagents. We were able to derivatize PGs with 9-anthryldiazomethtane (ADAM) at 4°C in the dark after 12 hours. This method was applied to analysis of human urine (20 μL). Clear separation and detection of PGs at fmole levels could be achieved. The lower detection limit was 1.0 fmole for PGF1α.",

author = "Shinya Sakae and Akira Harata and Takehiko Kitamori and Tsuguo Sawada and Akira Okubo and Shozo Toda and Takao Shimizu and Hideo Watanabe",

year = "1991",

month = jan,

doi = "10.2116/analsci.7.Supple_957",

language = "English",

volume = "7",

pages = "957--958",

journal = "Analytical Sciences",

issn = "0910-6340",

publisher = "The Japan Society for Analytical Chemistry",

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T1 - Highly Sensitive Simultaneous Determination of Prostaglandins by Using HPLC/Laser Induced Fluorescence (LIF).

AU - Sakae, Shinya

AU - Harata, Akira

AU - Kitamori, Takehiko

AU - Sawada, Tsuguo

AU - Okubo, Akira

AU - Toda, Shozo

AU - Shimizu, Takao

AU - Watanabe, Hideo

PY - 1991/1

Y1 - 1991/1

N2 - We have developed a laser induced fluorometric (LIF) detection method combined with HPLC and an analytical procedure for prostaglandins (PGs) which allows analysis of practical samples without interference from admixtures and residual reagents. We were able to derivatize PGs with 9-anthryldiazomethtane (ADAM) at 4°C in the dark after 12 hours. This method was applied to analysis of human urine (20 μL). Clear separation and detection of PGs at fmole levels could be achieved. The lower detection limit was 1.0 fmole for PGF1α.

AB - We have developed a laser induced fluorometric (LIF) detection method combined with HPLC and an analytical procedure for prostaglandins (PGs) which allows analysis of practical samples without interference from admixtures and residual reagents. We were able to derivatize PGs with 9-anthryldiazomethtane (ADAM) at 4°C in the dark after 12 hours. This method was applied to analysis of human urine (20 μL). Clear separation and detection of PGs at fmole levels could be achieved. The lower detection limit was 1.0 fmole for PGF1α.

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DO - 10.2116/analsci.7.Supple_957

M3 - Article

AN - SCOPUS:85004245853

SN - 0910-6340

VL - 7

SP - 957

EP - 958

JO - Analytical Sciences

JF - Analytical Sciences

ER -

Highly Sensitive Simultaneous Determination of Prostaglandins by Using HPLC/Laser Induced Fluorescence (LIF).

Abstract

All Science Journal Classification (ASJC) codes

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