Highly sensitive surface plasmon resonance sensor on nanoscale bioactive surfaces for specific detection of tri-nitro toluene

Praveen Singh, Takeshi Onodera, Kiyoshi Matsumoto, Norio Miura, Kiyoshi Toko

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

A nano-scale biosensor chip surface was fabricated using dinitro-phenylated keyhole limpet hemocyanin (DNP-KLH) protein conjugate as ligand supported by underlying 11-amino 1-undecanethiol hydrochloride(AUT) self-assembled monolayer (SAM) and bis sulfosuccinimidyl suberate (BS3) as crosslinker, Bioactive thin films were fabricated over gold chip via layer-by-layer self- assembly methods. Biomolecular interaction between substrate specific anti-TNT antibody and DNP-KLH conjugate surface was monitored through surface plasmon resonance based optical sensor. The quantitation of tri-nitro toluene(TNT) on this bioactive surface was done using the solution based competitive inhibition assay. The DNP-KLH surface biosensor has shown a detection limit of 0.14 ng/ml (140 ppt) for TNT molecule. The detection limit of surface plasmon resonance (SPR) biosensor was further enhanced by using goat anti mouse antibody to the 0.002 ng/ml for TNT analyte. This TNT specific biosensor holds the promise to be one of most sensitive sensor surface under indirect competitive assay format, A short injection (12 sec) of 10 mM glycine-HCl solution was found adequate for regeneration of DNP-KLH surface for repeated use.

Original languageEnglish
Title of host publicationNanofunctional Materials, Nanostructures and Novel Devices for Biological and Chemical Detection
PublisherMaterials Research Society
Pages273-280
Number of pages8
ISBN (Print)9781604234077
DOIs
Publication statusPublished - 2006
Event2006 MRS Fall Meeting - Boston, MA, United States
Duration: Nov 27 2006Dec 1 2006

Publication series

NameMaterials Research Society Symposium Proceedings
Volume951
ISSN (Print)0272-9172

Other

Other2006 MRS Fall Meeting
CountryUnited States
CityBoston, MA
Period11/27/0612/1/06

All Science Journal Classification (ASJC) codes

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

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    Singh, P., Onodera, T., Matsumoto, K., Miura, N., & Toko, K. (2006). Highly sensitive surface plasmon resonance sensor on nanoscale bioactive surfaces for specific detection of tri-nitro toluene. In Nanofunctional Materials, Nanostructures and Novel Devices for Biological and Chemical Detection (pp. 273-280). (Materials Research Society Symposium Proceedings; Vol. 951). Materials Research Society. https://doi.org/10.1557/proc-0951-e07-12