Histone Acetyltransferase Inhibitory Activity of Bokbunja (Rubus coreanus Miq.) ethanol extract on androgen receptor-dependent prostate cancer cell growth

Mi Jeong Kim, Ah Reum Seong, Yoo Hyun Lee, Young Jun Kim, Masaki Shiota, Akira Yokomizo, Seiji Naito, Jeongmin Lee, Woojin Jun, Ho Geun Yoon

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1 Citation (Scopus)

Abstract

In this study, we demonstrate that a bokbunja (Rubus coreanus) ethanol extract (RCE) exhibits the strong histone acetyltransferase (HAT) inhibitory activity, and shows specificity against the p300 HAT enzyme. RCE specifically inhibited p300 acetyltransferase activities with an IC50 of approximately 70 μg/mL, but did not inhibit other epigenetic enzymes. We found that RCE inhibited agonist-dependent androgen receptor (AR) acetylation and suppressed androgen-induced AR transcriptional activity. RCE treatment also decreased the enhancement of AR transcriptional activity caused by p300 overexpression, and combined treatment with RCE potentiated the activity of the AR antagonist flutamide. Finally, RCE treatment reduced the growth of LNCaP human prostate cancer cells via inhibition of cyclin D1 and cyclin E expression, and concomitantly induced apoptosis. Collectively, our results suggest that therapeutic targeting of AR acetylation by HATi could lead to a new class of antagonists for the treatment of prostate cancer.

Original languageEnglish
Pages (from-to)1503-1511
Number of pages9
JournalFood Science and Biotechnology
Volume19
Issue number6
DOIs
Publication statusPublished - 2010

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology

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