Histone deacetylase inhibitor trichostatin a induces cell-cycle arrest/apoptosis and hepatocyte differentiation in human hepatoma cells

Yo ichi Yamashita, Mitsuo Shimada, Norifumi Harimoto, Tatsuya Rikimaru, Ken Shirabe, Shinj Tanaka, Keizo Sugimachi

Research output: Contribution to journalArticle

137 Citations (Scopus)

Abstract

Remodeling of the chromatin template by inhibition of HDAC activities represents a potential transcriptional therapy for neoplastic disease. A number of HDAC inhibitors that modulate in vitro cell growth and differentiation have been developed. We analyzed the effects of TSA, a specific and potent HDAC inhibitor, on the human hepatoma cell lines HepG2 and Huh-7. TSA increased levels of acetylated histones H3 and H4 in both HepG2 and Huh-7. It inhibited cell proliferation in vitro and induced G0/G1 arrest in HepG2 and apoptosis in Huh-7. Gene expression of liver-specific functions and liver-enriched transcription factors was upregulated by TSA. TSA upregulated the ammonia removal rate and the albumin synthesis rate of HepG2 and Huh-7. Our results indicate that TSA can induce cell-cycle arrest/apoptosis and hepatocyte differentiation in human liver cancer cell lines.

Original languageEnglish
Pages (from-to)572-576
Number of pages5
JournalInternational Journal of Cancer
Volume103
Issue number5
DOIs
Publication statusPublished - Feb 20 2003

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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