Histone H3K9 Methyltransferase G9a in Oocytes Is Essential for Preimplantation Development but Dispensable for CG Methylation Protection

Wan Kin Au Yeung, Julie Brind'Amour, Yu Hatano, Kazuo Yamagata, Robert Feil, Matthew C. Lorincz, Makoto Tachibana, Yoichi Shinkai, Hiroyuki Sasaki

Research output: Contribution to journalArticle

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Abstract

Mammalian histone methyltransferase G9a (also called EHMT2) deposits H3K9me2 on chromatin and is essential for postimplantation development. However, its role in oogenesis and preimplantation development remains poorly understood. We show that H3K9me2-enriched chromatin domains in mouse oocytes are generally depleted of CG methylation, contrasting with their association in embryonic stem and somatic cells. Oocyte-specific disruption of G9a results in reduced H3K9me2 enrichment and impaired reorganization of heterochromatin in oocytes, but only a modest reduction in CG methylation is detected. Furthermore, in both oocytes and 2-cell embryos, G9a depletion has limited impact on the expression of genes and retrotransposons. Although their CG methylation is minimally affected, preimplantation embryos derived from such oocytes show abnormal chromosome segregation and frequent developmental arrest. Our findings illuminate the functional importance of G9a independent of CG methylation in preimplantation development and call into question the proposed role for H3K9me2 in CG methylation protection in zygotes. Au Yeung et al. report that H3K9 methyltransferase G9a in mouse oocytes is essential for preimplantation development. Contrary to the previous model, however, maternal G9a is dispensable for CG methylation protection in zygotes and is instead important for chromatin reorganization in oocytes and proper chromosome segregation in preimplantation embryos.

Original languageEnglish
Pages (from-to)282-293.e4
JournalCell Reports
Volume27
Issue number1
DOIs
Publication statusPublished - Apr 2 2019

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Methylation
Oocytes
Chromatin
Chromosome Segregation
Chromosomes
Zygote
Blastocyst
Retroelements
Methyltransferases
Oogenesis
Heterochromatin
Embryonic Stem Cells
histone methyltransferase
Deposits
Genes
Association reactions
Embryonic Structures
Mothers
Gene Expression

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Histone H3K9 Methyltransferase G9a in Oocytes Is Essential for Preimplantation Development but Dispensable for CG Methylation Protection. / Au Yeung, Wan Kin; Brind'Amour, Julie; Hatano, Yu; Yamagata, Kazuo; Feil, Robert; Lorincz, Matthew C.; Tachibana, Makoto; Shinkai, Yoichi; Sasaki, Hiroyuki.

In: Cell Reports, Vol. 27, No. 1, 02.04.2019, p. 282-293.e4.

Research output: Contribution to journalArticle

Au Yeung, WK, Brind'Amour, J, Hatano, Y, Yamagata, K, Feil, R, Lorincz, MC, Tachibana, M, Shinkai, Y & Sasaki, H 2019, 'Histone H3K9 Methyltransferase G9a in Oocytes Is Essential for Preimplantation Development but Dispensable for CG Methylation Protection', Cell Reports, vol. 27, no. 1, pp. 282-293.e4. https://doi.org/10.1016/j.celrep.2019.03.002
Au Yeung, Wan Kin ; Brind'Amour, Julie ; Hatano, Yu ; Yamagata, Kazuo ; Feil, Robert ; Lorincz, Matthew C. ; Tachibana, Makoto ; Shinkai, Yoichi ; Sasaki, Hiroyuki. / Histone H3K9 Methyltransferase G9a in Oocytes Is Essential for Preimplantation Development but Dispensable for CG Methylation Protection. In: Cell Reports. 2019 ; Vol. 27, No. 1. pp. 282-293.e4.
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