HlyF produced by extraintestinal pathogenic Escherichia coli is a virulence factor that regulates outer membrane vesicle biogenesis

Kazunori Murase, Patricia Martin, Gaëlle Porcheron, Sébastien Houle, Emmanuelle Helloin, Marie Pénary, Jean Philippe Nougayrède, Charles M. Dozois, Tetsuya Hayashi, Eric Oswald

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Escherichia coli can cause extraintestinal infections in humans and animals. The hlyF gene is epidemiologically associated with virulent strains of avian pathogenic E. coli and human neonatal meningitis-associated E. coli. We demonstrated that culture supernatants of E. coli expressing HlyF induced autophagy in eukaryotic cells. This phenotype coincided with an enhanced production of outer membrane vesicles (OMVs) by bacteria expressing HlyF. The HlyF protein displays a predicted catalytic domain of the shortchain dehydrogenase/reductase superfamily. This conserved domain was involved the ability of HlyF to promote the production of OMVs. The increased production of OMVs was associated with the release of toxins. hlyF was shown to be expressed during extraintestinal infection and to play a role in the virulence of extraintestinal pathogenic E. coli in a chicken model of colibacillosis. This is the first evidence that pathogenic bacteria produce a virulence factor directly involved in the production of OMVs.

Original languageEnglish
Pages (from-to)856-865
Number of pages10
JournalJournal of Infectious Diseases
Volume212
Issue number11
DOIs
Publication statusPublished - Jan 1 2016
Externally publishedYes

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Virulence Factors
Membranes
Escherichia coli
Escherichia coli Meningitis
Oxidoreductases
Bacteria
Autophagy
Eukaryotic Cells
Infection
Virulence
Chickens
Catalytic Domain
Phenotype
Extraintestinal Pathogenic Escherichia coli
Genes
Proteins

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Infectious Diseases

Cite this

HlyF produced by extraintestinal pathogenic Escherichia coli is a virulence factor that regulates outer membrane vesicle biogenesis. / Murase, Kazunori; Martin, Patricia; Porcheron, Gaëlle; Houle, Sébastien; Helloin, Emmanuelle; Pénary, Marie; Nougayrède, Jean Philippe; Dozois, Charles M.; Hayashi, Tetsuya; Oswald, Eric.

In: Journal of Infectious Diseases, Vol. 212, No. 11, 01.01.2016, p. 856-865.

Research output: Contribution to journalArticle

Murase, K, Martin, P, Porcheron, G, Houle, S, Helloin, E, Pénary, M, Nougayrède, JP, Dozois, CM, Hayashi, T & Oswald, E 2016, 'HlyF produced by extraintestinal pathogenic Escherichia coli is a virulence factor that regulates outer membrane vesicle biogenesis', Journal of Infectious Diseases, vol. 212, no. 11, pp. 856-865. https://doi.org/10.1093/infdis/jiv506
Murase, Kazunori ; Martin, Patricia ; Porcheron, Gaëlle ; Houle, Sébastien ; Helloin, Emmanuelle ; Pénary, Marie ; Nougayrède, Jean Philippe ; Dozois, Charles M. ; Hayashi, Tetsuya ; Oswald, Eric. / HlyF produced by extraintestinal pathogenic Escherichia coli is a virulence factor that regulates outer membrane vesicle biogenesis. In: Journal of Infectious Diseases. 2016 ; Vol. 212, No. 11. pp. 856-865.
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