Hollow fiber bioreactor perfusion culture system for magnetic force-based skeletal muscle tissue engineering

Yasunori Yamamoto, Akira Ito, Hideaki Jitsunobu, Katsuya Yamaguchi, Kawabe Yoshinori, Hiroshi Mizumoto, Masamichi Kamihira

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Large-scale skeletal muscle tissue cultures are often limited by nutrient supplementation and oxygen diffusion. In the present study, we used a hollow-fiber bioreactor system to supply nutrients and oxygen for the cultivation of high cell-density skeletal muscle tissue constructs fabricated by a magnetic force-based tissue engineering technique. C2C12 cells, magnetically-labeled with magnetite cationic liposomes (MCLs), were mixed with a type I collagen solution and seeded into the cell culture space of the hollow-fiber bioreactor. A magnet was then placed underneath the bioreactor to accumulate MCL-labeled cells in the space between the hollow fibers by magnetic force. Perfusion culture was performed using a myogenic differentiation medium for 7 d. Histological observation revealed that high cell-dense and viable tissue constructs containing myotubes were successfully formed. Furthermore, muscle-specific proteins, such as myosin heavy chain and tropomyosin, were detected by western blot, indicating that C2C12 cells underwent myogenic differentiation. These findings indicate that the hollow-fiber bioreactor system is an effective approach for the in vitro culture of large skeletal muscle tissue constructs, fabricated by magnetic force-based tissue engineering. &copy 2012 The Society of Chemical Engineer, Japan.

Original languageEnglish
Pages (from-to)348-354
Number of pages7
JournalJOURNAL OF CHEMICAL ENGINEERING OF JAPAN
Volume45
Issue number5
DOIs
Publication statusPublished - Jan 1 2012

Fingerprint

Bioreactors
Tissue engineering
Muscle
Ferrosoferric Oxide
Fibers
Liposomes
Magnetite
Tissue
Nutrients
Oxygen
Tissue culture
Tropomyosin
Myosin Heavy Chains
Collagen Type I
Cell culture
Collagen
Magnets
Proteins
Engineers

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

Hollow fiber bioreactor perfusion culture system for magnetic force-based skeletal muscle tissue engineering. / Yamamoto, Yasunori; Ito, Akira; Jitsunobu, Hideaki; Yamaguchi, Katsuya; Yoshinori, Kawabe; Mizumoto, Hiroshi; Kamihira, Masamichi.

In: JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, Vol. 45, No. 5, 01.01.2012, p. 348-354.

Research output: Contribution to journalArticle

@article{04ba9167d816469c9a6d5d0a2d9d8444,
title = "Hollow fiber bioreactor perfusion culture system for magnetic force-based skeletal muscle tissue engineering",
abstract = "Large-scale skeletal muscle tissue cultures are often limited by nutrient supplementation and oxygen diffusion. In the present study, we used a hollow-fiber bioreactor system to supply nutrients and oxygen for the cultivation of high cell-density skeletal muscle tissue constructs fabricated by a magnetic force-based tissue engineering technique. C2C12 cells, magnetically-labeled with magnetite cationic liposomes (MCLs), were mixed with a type I collagen solution and seeded into the cell culture space of the hollow-fiber bioreactor. A magnet was then placed underneath the bioreactor to accumulate MCL-labeled cells in the space between the hollow fibers by magnetic force. Perfusion culture was performed using a myogenic differentiation medium for 7 d. Histological observation revealed that high cell-dense and viable tissue constructs containing myotubes were successfully formed. Furthermore, muscle-specific proteins, such as myosin heavy chain and tropomyosin, were detected by western blot, indicating that C2C12 cells underwent myogenic differentiation. These findings indicate that the hollow-fiber bioreactor system is an effective approach for the in vitro culture of large skeletal muscle tissue constructs, fabricated by magnetic force-based tissue engineering. &copy 2012 The Society of Chemical Engineer, Japan.",
author = "Yasunori Yamamoto and Akira Ito and Hideaki Jitsunobu and Katsuya Yamaguchi and Kawabe Yoshinori and Hiroshi Mizumoto and Masamichi Kamihira",
year = "2012",
month = "1",
day = "1",
doi = "10.1252/jcej.11we237",
language = "English",
volume = "45",
pages = "348--354",
journal = "Journal of Chemical Engineering of Japan",
issn = "0021-9592",
publisher = "Society of Chemical Engineers, Japan",
number = "5",

}

TY - JOUR

T1 - Hollow fiber bioreactor perfusion culture system for magnetic force-based skeletal muscle tissue engineering

AU - Yamamoto, Yasunori

AU - Ito, Akira

AU - Jitsunobu, Hideaki

AU - Yamaguchi, Katsuya

AU - Yoshinori, Kawabe

AU - Mizumoto, Hiroshi

AU - Kamihira, Masamichi

PY - 2012/1/1

Y1 - 2012/1/1

N2 - Large-scale skeletal muscle tissue cultures are often limited by nutrient supplementation and oxygen diffusion. In the present study, we used a hollow-fiber bioreactor system to supply nutrients and oxygen for the cultivation of high cell-density skeletal muscle tissue constructs fabricated by a magnetic force-based tissue engineering technique. C2C12 cells, magnetically-labeled with magnetite cationic liposomes (MCLs), were mixed with a type I collagen solution and seeded into the cell culture space of the hollow-fiber bioreactor. A magnet was then placed underneath the bioreactor to accumulate MCL-labeled cells in the space between the hollow fibers by magnetic force. Perfusion culture was performed using a myogenic differentiation medium for 7 d. Histological observation revealed that high cell-dense and viable tissue constructs containing myotubes were successfully formed. Furthermore, muscle-specific proteins, such as myosin heavy chain and tropomyosin, were detected by western blot, indicating that C2C12 cells underwent myogenic differentiation. These findings indicate that the hollow-fiber bioreactor system is an effective approach for the in vitro culture of large skeletal muscle tissue constructs, fabricated by magnetic force-based tissue engineering. &copy 2012 The Society of Chemical Engineer, Japan.

AB - Large-scale skeletal muscle tissue cultures are often limited by nutrient supplementation and oxygen diffusion. In the present study, we used a hollow-fiber bioreactor system to supply nutrients and oxygen for the cultivation of high cell-density skeletal muscle tissue constructs fabricated by a magnetic force-based tissue engineering technique. C2C12 cells, magnetically-labeled with magnetite cationic liposomes (MCLs), were mixed with a type I collagen solution and seeded into the cell culture space of the hollow-fiber bioreactor. A magnet was then placed underneath the bioreactor to accumulate MCL-labeled cells in the space between the hollow fibers by magnetic force. Perfusion culture was performed using a myogenic differentiation medium for 7 d. Histological observation revealed that high cell-dense and viable tissue constructs containing myotubes were successfully formed. Furthermore, muscle-specific proteins, such as myosin heavy chain and tropomyosin, were detected by western blot, indicating that C2C12 cells underwent myogenic differentiation. These findings indicate that the hollow-fiber bioreactor system is an effective approach for the in vitro culture of large skeletal muscle tissue constructs, fabricated by magnetic force-based tissue engineering. &copy 2012 The Society of Chemical Engineer, Japan.

UR - http://www.scopus.com/inward/record.url?scp=84861581772&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84861581772&partnerID=8YFLogxK

U2 - 10.1252/jcej.11we237

DO - 10.1252/jcej.11we237

M3 - Article

VL - 45

SP - 348

EP - 354

JO - Journal of Chemical Engineering of Japan

JF - Journal of Chemical Engineering of Japan

SN - 0021-9592

IS - 5

ER -