Hollow fiber bioreactor perfusion culture system for magnetic force-based skeletal muscle tissue engineering

Yasunori Yamamoto, Akira Ito, Hideaki Jitsunobu, Katsuya Yamaguchi, Kawabe Yoshinori, Hiroshi Mizumoto, Masamichi Kamihira

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Abstract

Large-scale skeletal muscle tissue cultures are often limited by nutrient supplementation and oxygen diffusion. In the present study, we used a hollow-fiber bioreactor system to supply nutrients and oxygen for the cultivation of high cell-density skeletal muscle tissue constructs fabricated by a magnetic force-based tissue engineering technique. C2C12 cells, magnetically-labeled with magnetite cationic liposomes (MCLs), were mixed with a type I collagen solution and seeded into the cell culture space of the hollow-fiber bioreactor. A magnet was then placed underneath the bioreactor to accumulate MCL-labeled cells in the space between the hollow fibers by magnetic force. Perfusion culture was performed using a myogenic differentiation medium for 7 d. Histological observation revealed that high cell-dense and viable tissue constructs containing myotubes were successfully formed. Furthermore, muscle-specific proteins, such as myosin heavy chain and tropomyosin, were detected by western blot, indicating that C2C12 cells underwent myogenic differentiation. These findings indicate that the hollow-fiber bioreactor system is an effective approach for the in vitro culture of large skeletal muscle tissue constructs, fabricated by magnetic force-based tissue engineering. &copy 2012 The Society of Chemical Engineer, Japan.

Original languageEnglish
Pages (from-to)348-354
Number of pages7
JournalJOURNAL OF CHEMICAL ENGINEERING OF JAPAN
Volume45
Issue number5
DOIs
Publication statusPublished - Jan 1 2012

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All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)

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