Homogeneous enzymatic reactions in ionic liquids with poly(ethylene glycol)-modified subtilisin

Kazunori Nakashima, Tatsuo Maruyama, Noriho Kamiya, Masahiro Goto

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Subtilisin Carlsberg was covalently modified with comb-shaped poly(ethylene glycol) (PM13). PM13-modified subtilisin (PM 13-Sub) was readily solubilized in three different ionic liquids (ILs), i.e., [Emim][Tf2N], [C2OC1mim][Tf 2N] and [C2OHmim][Tf2N]. Analysis of homogeneous enzymatic reactions in the ILs revealed that PM13-Sub exhibited excellent catalytic performance while the native enzyme suspended in ILs showed no activity. Hydrophobicity of ILs slightly affected enzyme activity, and the relatively hydrophobic IL [Emim][Tf2N] was the preferred medium for enzymatic reactions, similar to enzymatic reactions in conventional organic solvents. Enzyme activity was much higher in [Emim][Tf2N] than in conventional organic solvents, and excellent activity was associated with unique properties of ILs such as hydrophobicity and high polarity. Furthermore, PM13-Sub showed good stability in [Emim][Tf 2N], and maintained 80% of its initial activity after 60 h.

Original languageEnglish
Pages (from-to)3462-3467
Number of pages6
JournalOrganic and Biomolecular Chemistry
Volume4
Issue number18
DOIs
Publication statusPublished - Sep 8 2006

Fingerprint

Subtilisin
Ionic Liquids
Ethylene Glycol
Polyethylene glycols
glycols
ethylene
liquids
enzyme activity
Enzyme activity
Hydrophobicity
hydrophobicity
Hydrophobic and Hydrophilic Interactions
Organic solvents
Enzymes
Subtilisins
Comb and Wattles
enzymes
polarity

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physical and Theoretical Chemistry
  • Organic Chemistry

Cite this

Homogeneous enzymatic reactions in ionic liquids with poly(ethylene glycol)-modified subtilisin. / Nakashima, Kazunori; Maruyama, Tatsuo; Kamiya, Noriho; Goto, Masahiro.

In: Organic and Biomolecular Chemistry, Vol. 4, No. 18, 08.09.2006, p. 3462-3467.

Research output: Contribution to journalArticle

@article{f42c2b44a1ea4019ba878e139826e1f2,
title = "Homogeneous enzymatic reactions in ionic liquids with poly(ethylene glycol)-modified subtilisin",
abstract = "Subtilisin Carlsberg was covalently modified with comb-shaped poly(ethylene glycol) (PM13). PM13-modified subtilisin (PM 13-Sub) was readily solubilized in three different ionic liquids (ILs), i.e., [Emim][Tf2N], [C2OC1mim][Tf 2N] and [C2OHmim][Tf2N]. Analysis of homogeneous enzymatic reactions in the ILs revealed that PM13-Sub exhibited excellent catalytic performance while the native enzyme suspended in ILs showed no activity. Hydrophobicity of ILs slightly affected enzyme activity, and the relatively hydrophobic IL [Emim][Tf2N] was the preferred medium for enzymatic reactions, similar to enzymatic reactions in conventional organic solvents. Enzyme activity was much higher in [Emim][Tf2N] than in conventional organic solvents, and excellent activity was associated with unique properties of ILs such as hydrophobicity and high polarity. Furthermore, PM13-Sub showed good stability in [Emim][Tf 2N], and maintained 80{\%} of its initial activity after 60 h.",
author = "Kazunori Nakashima and Tatsuo Maruyama and Noriho Kamiya and Masahiro Goto",
year = "2006",
month = "9",
day = "8",
doi = "10.1039/b608920h",
language = "English",
volume = "4",
pages = "3462--3467",
journal = "Organic and Biomolecular Chemistry",
issn = "1477-0520",
publisher = "Royal Society of Chemistry",
number = "18",

}

TY - JOUR

T1 - Homogeneous enzymatic reactions in ionic liquids with poly(ethylene glycol)-modified subtilisin

AU - Nakashima, Kazunori

AU - Maruyama, Tatsuo

AU - Kamiya, Noriho

AU - Goto, Masahiro

PY - 2006/9/8

Y1 - 2006/9/8

N2 - Subtilisin Carlsberg was covalently modified with comb-shaped poly(ethylene glycol) (PM13). PM13-modified subtilisin (PM 13-Sub) was readily solubilized in three different ionic liquids (ILs), i.e., [Emim][Tf2N], [C2OC1mim][Tf 2N] and [C2OHmim][Tf2N]. Analysis of homogeneous enzymatic reactions in the ILs revealed that PM13-Sub exhibited excellent catalytic performance while the native enzyme suspended in ILs showed no activity. Hydrophobicity of ILs slightly affected enzyme activity, and the relatively hydrophobic IL [Emim][Tf2N] was the preferred medium for enzymatic reactions, similar to enzymatic reactions in conventional organic solvents. Enzyme activity was much higher in [Emim][Tf2N] than in conventional organic solvents, and excellent activity was associated with unique properties of ILs such as hydrophobicity and high polarity. Furthermore, PM13-Sub showed good stability in [Emim][Tf 2N], and maintained 80% of its initial activity after 60 h.

AB - Subtilisin Carlsberg was covalently modified with comb-shaped poly(ethylene glycol) (PM13). PM13-modified subtilisin (PM 13-Sub) was readily solubilized in three different ionic liquids (ILs), i.e., [Emim][Tf2N], [C2OC1mim][Tf 2N] and [C2OHmim][Tf2N]. Analysis of homogeneous enzymatic reactions in the ILs revealed that PM13-Sub exhibited excellent catalytic performance while the native enzyme suspended in ILs showed no activity. Hydrophobicity of ILs slightly affected enzyme activity, and the relatively hydrophobic IL [Emim][Tf2N] was the preferred medium for enzymatic reactions, similar to enzymatic reactions in conventional organic solvents. Enzyme activity was much higher in [Emim][Tf2N] than in conventional organic solvents, and excellent activity was associated with unique properties of ILs such as hydrophobicity and high polarity. Furthermore, PM13-Sub showed good stability in [Emim][Tf 2N], and maintained 80% of its initial activity after 60 h.

UR - http://www.scopus.com/inward/record.url?scp=33748266666&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33748266666&partnerID=8YFLogxK

U2 - 10.1039/b608920h

DO - 10.1039/b608920h

M3 - Article

VL - 4

SP - 3462

EP - 3467

JO - Organic and Biomolecular Chemistry

JF - Organic and Biomolecular Chemistry

SN - 1477-0520

IS - 18

ER -