Homogeneous immunoassay based on dihexadecyl-dimethylammonium bilayer membranes

Hirotaka Ihara, Takashi Imamura, Toyoki Kunitake, Chuichi Hirayama

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We describe a rapid, highly sensitive, simple immunoassay based on cationic bilayer membranes prepared from dihexadecyl-dimethylammonium bromide. This detergent was dispersed in water to give a slightly turbid solution which contained lamellar (not vesicular) bilayer membranes with a gel to liquid crystal phase transition (Tc, 28°C). An anionic hapten (N,N′-bis(2,4-dinitrophenyl)-l-lysine) combined electrostatically with this cationic membrane to induce aggregation of membranes, making the solution turbid. This aggregation was suppressed by antibodies to the hapten. The aggregation rate was rapid at a temperature above Tc, and the amount of antibodies was quantified by measuring absorbance or light scattering of the aqueous solution with a detection limit of 0.1 ng/ml of the antibodies. Anionic and zwitterionic bilayer membranes from di(tetradecyl)phosphate and phosphocholine derivatives, respectively, were less sensitive as detectors of the hapten-antibody reaction. Ionic interactions between cationic bilayer membranes and anionic haptens are important for successful assay performance.

Original languageEnglish
Pages (from-to)51-57
Number of pages7
JournalClinica Chimica Acta
Volume175
Issue number1
DOIs
Publication statusPublished - Jun 30 1988

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

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