Homogeneous immunoassay based on dihexadecyl-dimethylammonium bilayer membranes

Hirotaka Ihara, Takashi Imamura, Toyoki Kunitake, Chuichi Hirayama

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We describe a rapid, highly sensitive, simple immunoassay based on cationic bilayer membranes prepared from dihexadecyl-dimethylammonium bromide. This detergent was dispersed in water to give a slightly turbid solution which contained lamellar (not vesicular) bilayer membranes with a gel to liquid crystal phase transition (Tc, 28°C). An anionic hapten (N,N′-bis(2,4-dinitrophenyl)-l-lysine) combined electrostatically with this cationic membrane to induce aggregation of membranes, making the solution turbid. This aggregation was suppressed by antibodies to the hapten. The aggregation rate was rapid at a temperature above Tc, and the amount of antibodies was quantified by measuring absorbance or light scattering of the aqueous solution with a detection limit of 0.1 ng/ml of the antibodies. Anionic and zwitterionic bilayer membranes from di(tetradecyl)phosphate and phosphocholine derivatives, respectively, were less sensitive as detectors of the hapten-antibody reaction. Ionic interactions between cationic bilayer membranes and anionic haptens are important for successful assay performance.

Original languageEnglish
Pages (from-to)51-57
Number of pages7
JournalClinica Chimica Acta
Volume175
Issue number1
DOIs
Publication statusPublished - Jun 30 1988

Fingerprint

Immunoassay
Haptens
Membranes
Agglomeration
Antibodies
Liquid Crystals
Phosphorylcholine
Phase Transition
Detergents
Light scattering
Lysine
Limit of Detection
Assays
Phase transitions
Gels
Phosphates
Derivatives
Detectors
Light
Temperature

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

Cite this

Homogeneous immunoassay based on dihexadecyl-dimethylammonium bilayer membranes. / Ihara, Hirotaka; Imamura, Takashi; Kunitake, Toyoki; Hirayama, Chuichi.

In: Clinica Chimica Acta, Vol. 175, No. 1, 30.06.1988, p. 51-57.

Research output: Contribution to journalArticle

Ihara, Hirotaka ; Imamura, Takashi ; Kunitake, Toyoki ; Hirayama, Chuichi. / Homogeneous immunoassay based on dihexadecyl-dimethylammonium bilayer membranes. In: Clinica Chimica Acta. 1988 ; Vol. 175, No. 1. pp. 51-57.
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