Honeycomb-like architecture produced by living bacteria, Gluconacetobacter xylinus

Yasumitsu Uraki, Junji Nemoto, Hiroyuki Otsuka, Yutaka Tamai, Junji Sugiyama, Takao Kishimoto, Makoto Ubukata, Hiroshi Yabu, Masaru Tanaka, Masatsugu Shimomura

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45 Citations (Scopus)

Abstract

Bacterial cellulose (BC)-producing bacterium, Gluconacetobacter xylinus (ATCC53582), was found to move along linear microgrooves of a stripe-patterned cellulosic scaffold. On the basis of this finding, fabrication of honeycomb-patterned BC was attempted by controlling the bacterial movement using a agarose film scaffold with honeycomb-patterned grooves (concave type). The patterned agarose film was prepared by three steps. The first was transcription of a honeycomb-patterned polycaprolactone film template with polydimethyl siloxane. When the bacteria were cultured on the scaffold under atmospheric conditions, only bacterial proliferation was observed. Honeycomb-patterned BC was obtained when cultured under a humid CO2 atmosphere. Electron diffraction and polarized microscopic observation showed that the patterned BC comprised of the well defined cellulose Iα microfibrils. As another attempt to fabricate honeycomb-patterned BC, the bacteria were cultured on the patterned cellulose and agarose film with convex type of honeycomb. This culture yielded no honeycomb-patterned BC. Therefore, concave type honeycomb scaffold is more suitable to fabricate honeycomb-patterned BC.

Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalCarbohydrate Polymers
Volume69
Issue number1
DOIs
Publication statusPublished - May 1 2007
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Organic Chemistry
  • Polymers and Plastics
  • Materials Chemistry

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    Uraki, Y., Nemoto, J., Otsuka, H., Tamai, Y., Sugiyama, J., Kishimoto, T., Ubukata, M., Yabu, H., Tanaka, M., & Shimomura, M. (2007). Honeycomb-like architecture produced by living bacteria, Gluconacetobacter xylinus. Carbohydrate Polymers, 69(1), 1-6. https://doi.org/10.1016/j.carbpol.2006.08.021