Host range specificity of a novel runaway-type vector for mammalian cells

Hideyo Kirinaka, Masamichi Kamihira, Shinji Iijima

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

For industrial applications of our novel runaway-type vector for mammalian cells based on simian virus 40, we studied host range specificity of this vector, especially with human-derived cell lines, since human cells as hosts seem to be very important for the production of pharmaceuticals by genetic engineering. The runaway-type vector showed a high replication rate at a permissive temperature of 33°C in HeLa cells and erythropoietin was produced effectively. The plasmid copy number was dependent on the temperature but was relatively low in KB cells. KB cells produced erythropoietin effectively at a non-permissive temperature of 37°C despite the low plasmid copy number. On the other hand, neither runaway-type replication nor erythropoietin production was observed with blood cell derivatives such as HL-60 and RPMI-8226 cells.

Original languageEnglish
Pages (from-to)360-362
Number of pages3
JournalJournal of Fermentation and Bioengineering
Volume79
Issue number4
DOIs
Publication statusPublished - Jan 1 1995
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

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