Abstract
For industrial applications of our novel runaway-type vector for mammalian cells based on simian virus 40, we studied host range specificity of this vector, especially with human-derived cell lines, since human cells as hosts seem to be very important for the production of pharmaceuticals by genetic engineering. The runaway-type vector showed a high replication rate at a permissive temperature of 33°C in HeLa cells and erythropoietin was produced effectively. The plasmid copy number was dependent on the temperature but was relatively low in KB cells. KB cells produced erythropoietin effectively at a non-permissive temperature of 37°C despite the low plasmid copy number. On the other hand, neither runaway-type replication nor erythropoietin production was observed with blood cell derivatives such as HL-60 and RPMI-8226 cells.
Original language | English |
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Pages (from-to) | 360-362 |
Number of pages | 3 |
Journal | Journal of Fermentation and Bioengineering |
Volume | 79 |
Issue number | 4 |
DOIs | |
Publication status | Published - Jan 1 1995 |
Externally published | Yes |
All Science Journal Classification (ASJC) codes
- Biotechnology
- Applied Microbiology and Biotechnology