Abstract
For industrial applications of our novel runaway-type vector for mammalian cells based on simian virus 40, we studied host range specificity of this vector, especially with human-derived cell lines, since human cells as hosts seem to be very important for the production of pharmaceuticals by genetic engineering. The runaway-type vector showed a high replication rate at a permissive temperature of 33°C in HeLa cells and erythropoietin was produced effectively. The plasmid copy number was dependent on the temperature but was relatively low in KB cells. KB cells produced erythropoietin effectively at a non-permissive temperature of 37°C despite the low plasmid copy number. On the other hand, neither runaway-type replication nor erythropoietin production was observed with blood cell derivatives such as HL-60 and RPMI-8226 cells.
| Original language | English |
|---|---|
| Pages (from-to) | 360-362 |
| Number of pages | 3 |
| Journal | Journal of Fermentation and Bioengineering |
| Volume | 79 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Jan 1 1995 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Biotechnology
- Applied Microbiology and Biotechnology
Cite this
Host range specificity of a novel runaway-type vector for mammalian cells. / Kirinaka, Hideyo; Kamihira, Masamichi; Iijima, Shinji.
In: Journal of Fermentation and Bioengineering, Vol. 79, No. 4, 01.01.1995, p. 360-362.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Host range specificity of a novel runaway-type vector for mammalian cells
AU - Kirinaka, Hideyo
AU - Kamihira, Masamichi
AU - Iijima, Shinji
PY - 1995/1/1
Y1 - 1995/1/1
N2 - For industrial applications of our novel runaway-type vector for mammalian cells based on simian virus 40, we studied host range specificity of this vector, especially with human-derived cell lines, since human cells as hosts seem to be very important for the production of pharmaceuticals by genetic engineering. The runaway-type vector showed a high replication rate at a permissive temperature of 33°C in HeLa cells and erythropoietin was produced effectively. The plasmid copy number was dependent on the temperature but was relatively low in KB cells. KB cells produced erythropoietin effectively at a non-permissive temperature of 37°C despite the low plasmid copy number. On the other hand, neither runaway-type replication nor erythropoietin production was observed with blood cell derivatives such as HL-60 and RPMI-8226 cells.
AB - For industrial applications of our novel runaway-type vector for mammalian cells based on simian virus 40, we studied host range specificity of this vector, especially with human-derived cell lines, since human cells as hosts seem to be very important for the production of pharmaceuticals by genetic engineering. The runaway-type vector showed a high replication rate at a permissive temperature of 33°C in HeLa cells and erythropoietin was produced effectively. The plasmid copy number was dependent on the temperature but was relatively low in KB cells. KB cells produced erythropoietin effectively at a non-permissive temperature of 37°C despite the low plasmid copy number. On the other hand, neither runaway-type replication nor erythropoietin production was observed with blood cell derivatives such as HL-60 and RPMI-8226 cells.
UR - http://www.scopus.com/inward/record.url?scp=0029035730&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029035730&partnerID=8YFLogxK
U2 - 10.1016/0922-338X(95)93995-V
DO - 10.1016/0922-338X(95)93995-V
M3 - Article
AN - SCOPUS:0029035730
VL - 79
SP - 360
EP - 362
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
SN - 1389-1723
IS - 4
ER -