Host range specificity of a novel runaway-type vector for mammalian cells

Hideyo Kirinaka, Masamichi Kamihira, Shinji Iijima

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

For industrial applications of our novel runaway-type vector for mammalian cells based on simian virus 40, we studied host range specificity of this vector, especially with human-derived cell lines, since human cells as hosts seem to be very important for the production of pharmaceuticals by genetic engineering. The runaway-type vector showed a high replication rate at a permissive temperature of 33°C in HeLa cells and erythropoietin was produced effectively. The plasmid copy number was dependent on the temperature but was relatively low in KB cells. KB cells produced erythropoietin effectively at a non-permissive temperature of 37°C despite the low plasmid copy number. On the other hand, neither runaway-type replication nor erythropoietin production was observed with blood cell derivatives such as HL-60 and RPMI-8226 cells.

Original languageEnglish
Pages (from-to)360-362
Number of pages3
JournalJournal of Fermentation and Bioengineering
Volume79
Issue number4
DOIs
Publication statusPublished - Jan 1 1995
Externally publishedYes

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Homeless Youth
Host Specificity
Erythropoietin
KB Cells
Cells
Temperature
Plasmids
Genetic Engineering
Simian virus 40
HeLa Cells
Genetic engineering
Blood Cells
Viruses
Drug products
Industrial applications
Cell Line
Blood
Derivatives
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Host range specificity of a novel runaway-type vector for mammalian cells. / Kirinaka, Hideyo; Kamihira, Masamichi; Iijima, Shinji.

In: Journal of Fermentation and Bioengineering, Vol. 79, No. 4, 01.01.1995, p. 360-362.

Research output: Contribution to journalArticle

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