HSP90α plays an important role in piRNA biogenesis and retrotransposon repression in mouse

Tomoko Ichiyanagi, Kenji Ichiyanagi, Ayako Ogawa, Satomi Kuramochi-Miyagawa, Toru Nakano, Shinichiro Chuma, Hiroyuki Sasaki, Heiichiro Udono

    Research output: Contribution to journalArticle

    18 Citations (Scopus)

    Abstract

    HSP90, found in all kingdoms of life, is a major chaperone protein regulating many client proteins. We demonstrated that HSP90α, one of two paralogs duplicated in vertebrates, plays an important role in the biogenesis of fetal PIWI-interacting RNAs (piRNA), which act against the transposon activities, in mouse male germ cells. The knockout mutation of Hsp90a resulted in a large reduction in the expression of primary and secondary piRNAs and mislocalization of MIWI2, a PIWI homolog. Whereas the mutation in Fkbp6 encoding a co-chaperone reduced piRNAs of 28-32 nucleotides in length, the Hsp90a mutation reduced piRNAs of 24-32 nucleotides, suggesting the presence of both FKBP6-dependent and -independent actions of HSP90α. Although DNA methylation and mRNA levels of L1 retrotransposon were largely unchanged in the Hsp90a mutant testes, the L1-encoded protein was increased, suggesting the presence of post-transcriptional regulation. This study revealed the specialized function of the HSP90α isofom in the piRNA biogenesis and repression of retrotransposons during the development of male germ cells in mammals.

    Original languageEnglish
    Pages (from-to)11903-11911
    Number of pages9
    JournalNucleic acids research
    Volume42
    Issue number19
    DOIs
    Publication statusPublished - Oct 29 2014

    Fingerprint

    Retroelements
    RNA
    Germ Cells
    Mutation
    Nucleotides
    Proteins
    DNA Methylation
    Vertebrates
    Testis
    Mammals
    Messenger RNA

    All Science Journal Classification (ASJC) codes

    • Genetics

    Cite this

    Ichiyanagi, T., Ichiyanagi, K., Ogawa, A., Kuramochi-Miyagawa, S., Nakano, T., Chuma, S., ... Udono, H. (2014). HSP90α plays an important role in piRNA biogenesis and retrotransposon repression in mouse. Nucleic acids research, 42(19), 11903-11911. https://doi.org/10.1093/nar/gku881

    HSP90α plays an important role in piRNA biogenesis and retrotransposon repression in mouse. / Ichiyanagi, Tomoko; Ichiyanagi, Kenji; Ogawa, Ayako; Kuramochi-Miyagawa, Satomi; Nakano, Toru; Chuma, Shinichiro; Sasaki, Hiroyuki; Udono, Heiichiro.

    In: Nucleic acids research, Vol. 42, No. 19, 29.10.2014, p. 11903-11911.

    Research output: Contribution to journalArticle

    Ichiyanagi, T, Ichiyanagi, K, Ogawa, A, Kuramochi-Miyagawa, S, Nakano, T, Chuma, S, Sasaki, H & Udono, H 2014, 'HSP90α plays an important role in piRNA biogenesis and retrotransposon repression in mouse', Nucleic acids research, vol. 42, no. 19, pp. 11903-11911. https://doi.org/10.1093/nar/gku881
    Ichiyanagi T, Ichiyanagi K, Ogawa A, Kuramochi-Miyagawa S, Nakano T, Chuma S et al. HSP90α plays an important role in piRNA biogenesis and retrotransposon repression in mouse. Nucleic acids research. 2014 Oct 29;42(19):11903-11911. https://doi.org/10.1093/nar/gku881
    Ichiyanagi, Tomoko ; Ichiyanagi, Kenji ; Ogawa, Ayako ; Kuramochi-Miyagawa, Satomi ; Nakano, Toru ; Chuma, Shinichiro ; Sasaki, Hiroyuki ; Udono, Heiichiro. / HSP90α plays an important role in piRNA biogenesis and retrotransposon repression in mouse. In: Nucleic acids research. 2014 ; Vol. 42, No. 19. pp. 11903-11911.
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