Human amniotic epithelial cells possess hepatocyte-like characteristic and functions

Seiji Takashima, Hirohiko Ise, Peng Zhao, Toshihiro Akaike, Toshio Nikaido

Research output: Contribution to journalArticle

118 Citations (Scopus)

Abstract

Hepatocyte transplantation is expected to become a novel method for treatment of liver disease. However, many questions remain regarding this approach, especially concerning donor cells. To evaluate whether human amniotic epithelial cells can be used as a cell source for hepatocyte transplantation, hepatic gene expression and functions of human amniotic epithelial cells were analyzed. Reverse transcription-polymerase chain reaction analysis demonstrated that human amniotic epithelial cells expressed albumin, α 1-antitrypsin, and other hepatocyte-related genes. Cultivated human amniotic epithelial cells demonstrated albumin production, glycogen storage, and albumin secretion consistent with the hepatocyte gene expression profile. In organ culture, the amnion secreted 30-fold larger amounts of albumin than human amniotic epithelial cells in monolayer culture. Moreover, in organ culture the amnion also secreted α1-antitrypsin. Following transplantation into mice, the amnion survived and secreted albumin. These observations suggest that transplantation of human amniotic epithelial cells and/or amnion could be novel therapeutic strategy for treatment of hepatic diseases, including α1-antitrypsin deficiency.

Original languageEnglish
Pages (from-to)73-84
Number of pages12
JournalCell structure and function
Volume29
Issue number3
DOIs
Publication statusPublished - Jun 1 2004
Externally publishedYes

Fingerprint

Hepatocytes
Amnion
Epithelial Cells
Albumins
Transplantation
Organ Culture Techniques
Glycogen
Transcriptome
Liver Transplantation
Reverse Transcription
Liver Diseases
Therapeutics
Gene Expression
Polymerase Chain Reaction
Liver
Genes

All Science Journal Classification (ASJC) codes

  • Physiology
  • Molecular Biology
  • Cell Biology

Cite this

Human amniotic epithelial cells possess hepatocyte-like characteristic and functions. / Takashima, Seiji; Ise, Hirohiko; Zhao, Peng; Akaike, Toshihiro; Nikaido, Toshio.

In: Cell structure and function, Vol. 29, No. 3, 01.06.2004, p. 73-84.

Research output: Contribution to journalArticle

Takashima, Seiji ; Ise, Hirohiko ; Zhao, Peng ; Akaike, Toshihiro ; Nikaido, Toshio. / Human amniotic epithelial cells possess hepatocyte-like characteristic and functions. In: Cell structure and function. 2004 ; Vol. 29, No. 3. pp. 73-84.
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