Human amniotic mesenchymal cells have some characteristics of cardiomyocytes

Peng Zhao, Hirohiko Ise, Minoru Hongo, Masao Ota, Ikuo Konishi, Toshio Nikaido

Research output: Contribution to journalArticle

202 Citations (Scopus)

Abstract

Background. Cellular cardiomyoplasty (CCM) is a major method for the treatment of heart failure because adult cardiomyocytes do not regenerate after ischemic injury, which results in heart failure. There is a great deal of interest in finding suitable new cell sources for use in CCM. Here, we report that human amniotic mesenchymal cells (hAMC), which are multipotent cells derived from fetal mesoderm, may be a suitable cell source for CCM. Methods. Freshly isolated hAMC were examined to detect the expression of cardiac-specific genes by reverse-transcription polymerase chain reaction and immunocytochemistry. hAMC were cocultivated with neonatal rat heart explants and transplanted into myocardial infarcts in the rat heart. Results. hAMC expressed cardiac-specific transcription factor GATA4, cardiac-specific genes, such as myosin light chain (MLC)-2a, MLC-2v, cTnI, and cTnT, and the α-subunits of the cardiac-specific L-type calcium channel (α1c) and the transient outward potassium channel (Kv4.3). After stimulation with basic fibroblast growth factor (bFGF) or activin A, hAMC expressed Nkx2.5, a specific transcription factor for the cardiomyocyte and cardiac-specific marker atrial natriuretic peptide. In addition, the cardiac-specific gene α-myosin heavy chain was detected after treatment with activin A. Coculture experiments confirmed that hAMC were able to both integrate into cardiac tissues and differentiate into cardiomyocyte-like cells. After transplantation into the myocardial infarcts in rat hearts, hAMC survived in the scar tissue for at least 2 months and differentiated into cardiomyocyte-like cells. Conclusion. The results of the present study suggest that hAMC possess some characteristics of cardiomyocytes.

Original languageEnglish
Pages (from-to)528-535
Number of pages8
JournalTransplantation
Volume79
Issue number5
DOIs
Publication statusPublished - Mar 15 2005

All Science Journal Classification (ASJC) codes

  • Transplantation

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