Human C-type natriuretic peptide

Yoshihiro Ogawa, Kazuwa Nakao, Osamu Nakagawa, Yasato Komatsu, Kiminori Hosoda, Shin Ichi Suga, Hiroshi Arai, Kiyoshi Nagata, Nobuo Yoshida, Hiroo Imura

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

We isolated the human C-type natriuretic peptide gene and identified the peptide in the brain. The human C-type natriuretic peptide gene appeared to be composed of at least two exons and one intron. In the 5′-flanking region, there is an array of cis elements (an inverted CCA AT box, two GC boxes, and a cyclic AMP response element-like sequence) that is not present in upstream sequences of the atrial and brain natriuretic peptide genes. Analysis of the deduced amino acid sequence revealed that human prepro C-type natriuretic peptide comprises 126 amino acids and that the C-terminal 22-residue peptide (G-L-S-K-G-C-F-G-L-K-L-D-R-I-G-S-M-S-G-L-G-C) preceded by Lys-Lys is identical to the porcine counterpart. However, replacement of two amino acids took place in the C-terminal 53-residue sequence, corresponding to another endogenous form of the peptide. Reverse-phase high-performance liquid chromatography coupled with a radioimmunoassay for C-type natriuretic peptide demonstrated that it occurs in the human brain. C-type natriuretic peptide–like immunoreactivity was detected in discrete regions of the human brain, and its level was 10-fold higher than the atrial and brain natriuretic peptide levels, raising the possibility that C-type natriuretic peptide is the major natriuretic peptide in the human brain.

Original languageEnglish
Pages (from-to)809-813
Number of pages5
JournalHypertension
Volume19
Issue number6
Publication statusPublished - Jan 1 1992
Externally publishedYes

Fingerprint

C-Type Natriuretic Peptide
Brain Natriuretic Peptide
Brain
Atrial Natriuretic Factor
lysyllysine
Genes
Amino Acids
Natriuretic Peptides
Peptides
5' Flanking Region
Protein Sequence Analysis
Response Elements
Reverse-Phase Chromatography
Cyclic AMP
Introns
Radioimmunoassay
Exons
Swine
High Pressure Liquid Chromatography

All Science Journal Classification (ASJC) codes

  • Internal Medicine

Cite this

Ogawa, Y., Nakao, K., Nakagawa, O., Komatsu, Y., Hosoda, K., Suga, S. I., ... Imura, H. (1992). Human C-type natriuretic peptide. Hypertension, 19(6), 809-813.

Human C-type natriuretic peptide. / Ogawa, Yoshihiro; Nakao, Kazuwa; Nakagawa, Osamu; Komatsu, Yasato; Hosoda, Kiminori; Suga, Shin Ichi; Arai, Hiroshi; Nagata, Kiyoshi; Yoshida, Nobuo; Imura, Hiroo.

In: Hypertension, Vol. 19, No. 6, 01.01.1992, p. 809-813.

Research output: Contribution to journalArticle

Ogawa, Y, Nakao, K, Nakagawa, O, Komatsu, Y, Hosoda, K, Suga, SI, Arai, H, Nagata, K, Yoshida, N & Imura, H 1992, 'Human C-type natriuretic peptide', Hypertension, vol. 19, no. 6, pp. 809-813.
Ogawa Y, Nakao K, Nakagawa O, Komatsu Y, Hosoda K, Suga SI et al. Human C-type natriuretic peptide. Hypertension. 1992 Jan 1;19(6):809-813.
Ogawa, Yoshihiro ; Nakao, Kazuwa ; Nakagawa, Osamu ; Komatsu, Yasato ; Hosoda, Kiminori ; Suga, Shin Ichi ; Arai, Hiroshi ; Nagata, Kiyoshi ; Yoshida, Nobuo ; Imura, Hiroo. / Human C-type natriuretic peptide. In: Hypertension. 1992 ; Vol. 19, No. 6. pp. 809-813.
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