Human complement factor B

cDNA cloning, nucleotide sequencing, phenotypic conversion by site-directed mutagenesis and expression

Takahiko Horiuchi, Kim Sunghee, Matsumoto Mitsuru, Watanabe Ichiro, Fujita Shigeru, John E. Volanakis

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

A full-length cDNA clone, BHL4-1, encoding factor B was isolated from a human liver cDNA library and sequenced in its entirety. It consists of 2388 bp which include a 5'-untranslated region of 40 bp, a single open reading frame, 2292 bp in length, and a 3'-untranslated region of 56 bp followed by a poly-A tail. The deduced amino acid sequence comprises 25 residues of a putative leader peptide and 739 residues of the mature polypeptide chain of the F allele of factor B. We constructed an S allele-like Q7R mutant of BHL4-1 by site-directed mutagenesis. Both the wild-type and mutant factor B cDNA were expressed transiently in a eukaryotic system. The specific hemolytic activities of the two recombinant factor B alleles and of native B were not significantly different from each other.

Original languageEnglish
Pages (from-to)1587-1592
Number of pages6
JournalMolecular Immunology
Volume30
Issue number17
DOIs
Publication statusPublished - Jan 1 1993
Externally publishedYes

Fingerprint

Complement Factor B
Site-Directed Mutagenesis
Organism Cloning
Nucleotides
Complementary DNA
Alleles
5' Untranslated Regions
3' Untranslated Regions
Protein Sorting Signals
Gene Library
Open Reading Frames
Amino Acid Sequence
Clone Cells
Messenger RNA
Peptides
Liver

All Science Journal Classification (ASJC) codes

  • Immunology
  • Molecular Biology

Cite this

Human complement factor B : cDNA cloning, nucleotide sequencing, phenotypic conversion by site-directed mutagenesis and expression. / Horiuchi, Takahiko; Sunghee, Kim; Mitsuru, Matsumoto; Ichiro, Watanabe; Shigeru, Fujita; Volanakis, John E.

In: Molecular Immunology, Vol. 30, No. 17, 01.01.1993, p. 1587-1592.

Research output: Contribution to journalArticle

Horiuchi, Takahiko ; Sunghee, Kim ; Mitsuru, Matsumoto ; Ichiro, Watanabe ; Shigeru, Fujita ; Volanakis, John E. / Human complement factor B : cDNA cloning, nucleotide sequencing, phenotypic conversion by site-directed mutagenesis and expression. In: Molecular Immunology. 1993 ; Vol. 30, No. 17. pp. 1587-1592.
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